P3H4 (AA 1-443) protein (His tag)
made-to-order
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target
- P3H4
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-443
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- His tag
- Purpose
- Custom-made recombinant P3h4 Protein expressed in mammalian cells.
- Sequence
- MARGAWGLLW LLLGSAGAQY EKYSFRGFPP EDLMPLATAY GHALEQYEGE SWRESARYLE AALRLHRLLR DSEAFCHANC SGPATSQPRP ASGPDGDHDG DGEDWARELR LFGHVLERAA CLRRCKKTLP AFQVPYPPRQ LLRDFQSRLP YQYLHYAHFK ANRLEKAVAA AYTFLQRNPK HELTAKYLNY YRGMLDIGDE SLTDLEAQPY EAVFLRAVKL YNSGDFRSST EDMERALAEY MTVFARCLAG CEGAHEQVDF KDFYPAIADL FAESLQCKVD CETNLTPNVG GFFVDKFVAT MYHYLQFAYY KLNDVRQAAR SAASYMLFDP KDSVMQQNLV YYRFHRARWG LEDEDFQPRE EAMLYHNQTS ELRELLDFTH MYLQSDDEME LEETESLPEP EEPLSDAEFE GEGDYEEGLY ADWWQEPEAK GDEAEAEPEP ELT Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- made-to-order
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P3H4 (AA 1-437) protein (His tag)
made-to-order Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- P3H4
- Background
- Endoplasmic reticulum protein SC65 (Leprecan-like protein 4) (Prolyl 3-hydroxylase family member 4) (Synaptonemal complex protein SC65),FUNCTION: Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linking of collagen fibrils (PubMed:27119146). Required for normal bone density and normal skin stability via its role in hydroxylation of lysine residues in collagen alpha chains and in collagen fibril assembly (PubMed:23959653, PubMed:27119146, PubMed:28115524). {ECO:0000269|PubMed:23959653, ECO:0000269|PubMed:27119146, ECO:0000269|PubMed:28115524}.
- Molecular Weight
- 51.1 kDa
- UniProt
- Q8K2B0
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