P3H4 (AA 1-443) protein (His tag)

Catalog No. ABIN7559499

Recombinant protein expressed in HEK-293 Cells.

Quick Overview for P3H4 (AA 1-443) protein (His tag) (ABIN7559499)

Target: P3H4

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-443

Purification tag / Conjugate: His tag

Purpose: Predefined custom protein recombinant P3h4 Protein expressed in mammalian cells.

Sequence: MARGAWGLLW LLLGSAGAQY EKYSFRGFPP EDLMPLATAY GHALEQYEGE SWRESARYLE AALRLHRLLR DSEAFCHANC SGPATSQPRP ASGPDGDHDG DGEDWARELR LFGHVLERAA CLRRCKKTLP AFQVPYPPRQ LLRDFQSRLP YQYLHYAHFK ANRLEKAVAA AYTFLQRNPK HELTAKYLNY YRGMLDIGDE SLTDLEAQPY EAVFLRAVKL YNSGDFRSST EDMERALAEY MTVFARCLAG CEGAHEQVDF KDFYPAIADL FAESLQCKVD CETNLTPNVG GFFVDKFVAT MYHYLQFAYY KLNDVRQAAR SAASYMLFDP KDSVMQQNLV YYRFHRARWG LEDEDFQPRE EAMLYHNQTS ELRELLDFTH MYLQSDDEME LEETESLPEP EEPLSDAEFE GEGDYEEGLY ADWWQEPEAK GDEAEAEPEP ELT Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: P3H4

Background: Endoplasmic reticulum protein SC65 (Leprecan-like protein 4) (Prolyl 3-hydroxylase family member 4) (Synaptonemal complex protein SC65),FUNCTION: Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linking of collagen fibrils (PubMed:27119146). Required for normal bone density and normal skin stability via its role in hydroxylation of lysine residues in collagen alpha chains and in collagen fibril assembly (PubMed:23959653, PubMed:27119146, PubMed:28115524). {ECO:0000269|PubMed:23959653, ECO:0000269|PubMed:27119146, ECO:0000269|PubMed:28115524}.

Molecular Weight: 51.1 kDa

UniProt: Q8K2B0

AlphaFold: Q8K2B0