GSPT1 Protein (AA 1-636) (His tag)

Catalog No. ABIN7559615

This Recombinant GSPT1 protein is expressed in HEK-293 Cells.

Quick Overview for GSPT1 Protein (AA 1-636) (His tag) (ABIN7559615)

Target: GSPT1 (G1 To S Phase Transition 1 (GSPT1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-636

Purification tag / Conjugate: This GSPT1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Gspt1 Protein expressed in mammalian cells.

Sequence: MDPSSGGGGG GGGGGSSSSS DSAPDCWDQT DMEAPGPGPC GGGGSGSGSM AAVAEAQREN LSAAFSRQLN VNAKPFVPNV HAAEFVPSFL RGPAQPPLSP AGAAGGDHGA GSGAGGPSEP VESSQDQSCE GSNSTVSMEL SEPVVENGET EMSPEESWEH KEEISEAEPG GGSSGDGRPP EESTQEMMEE EEEIPKPKSA VAPPGAPKKE HVNVVFIGHV DAGKSTIGGQ IMYLTGMVDK RTLEKYEREA KEKNRETWYL SWALDTNQEE RDKGKTVEVG RAYFETEKKH FTILDAPGHK SFVPNMIGGA SQADLAVLVI SARKGEFETG FEKGGQTREH AMLAKTAGVK HLIVLINKMD DPTVNWSNER YEECKEKLVP FLKKVGFNPK KDIHFMPCSG LTGANLKEQS DFCPWYIGLP FIPYLDNLPN FNRSVDGPIR LPIVDKYKDM GTVVLGKLES GSICKGQQLV MMPNKHNVEV LGILSDDVET DSVAPGENLK IRLKGIEEEE ILPGFILCDL NNLCHSGRTF DAQIVIIEHK SIICPGYNAV LHIHTCIEEV EITALICLVD KKSGEKSKTR PRFVKQDQVC IARLRTAGTI CLETFKDFPQ MGRFTLRDEG KTIAIGKVLK LVPEKD Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: GSPT1 (G1 To S Phase Transition 1 (GSPT1))

Alternative Name: Gspt1

Background: Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (Eukaryotic peptide chain release factor subunit 3a) (eRF3a) (EC 3.6.5.-) (G1 to S phase transition protein 1 homolog),FUNCTION: GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA. GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes. {ECO:0000250|UniProtKB:P15170}.

Molecular Weight: 68.6 kDa

UniProt: Q8R050

AlphaFold: Q8R050