POMT1 Protein (AA 1-746) (His tag)

Catalog No. ABIN7559700

Recombinant POMT1 protein expressed in HEK-293 Cells.

Quick Overview for POMT1 Protein (AA 1-746) (His tag) (ABIN7559700)

Target: POMT1 (Protein-O-Mannosyltransferase 1 (POMT1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-746

Purification tag / Conjugate: This POMT1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Pomt1 Protein expressed in mammalian cells.

Sequence: MGSHSTGLEE TLGVLPSWLF CKMLRFLKRP LVVTVDINLN LVALTGLGLL TRLWQLSYPR AVVFDEVYYG QYISFYMKRI FFLDDSGPPF GHMLLALGGW LGGFDGNFLW NRIGAEYSSN VPIWSLRLLP ALAGALSVPM AYQIVLELHF SHGAAIGAAL LMLIENALIT QSRLMLLESI LIFFNLLAVL SYLKFFNSQT HSPFSVHWWL WLLLTGVSCS CAVGIKYMGI FTYLLVLGIA AVHAWNLIGD QTLSNMRVLS HLLARIVALL VVPVFLYLLF FYVHLMLLYR SGPHDQIMSS AFQASLEGGL ARITQGQPLE VAFGSQVTLK SVSGKPLPCW LHSHKNTYPM IYENGRGSSH QQQVTCYPFK DINNWWIVKD PGRHQLVVNN PPRPVRHGDI VQLVHGMTTR LLNTHDVAAP LSPHSQEVSC YIDYNISMPA QNLWKLDIVN RESNRDTWKT ILSEVRFVHV NTSAILKLSG AHLPDWGFRQ LEVVGEKLSP GYHESMVWNV EEHRYGKSHE QKERELELHS PTQLDISRNL SFMARFSELQ WKMLTLKNED LEHQYSSTPL EWLTLDTNIA YWLHPRTSAQ IHLLGNIVIW TSASLATVVY TLLFFWYLLR RRRSICDLPE DAWSRWVLAG ALCTGGWALN YLPFFLMERV LFLYHYLPAL TFQILLLPIV LQHASDHLCR SQLQRNVFSA LVVAWYSSAC HVSNMLRPLT YGDTSLSPGE LRALRWKDSW DILIRK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: POMT1 (Protein-O-Mannosyltransferase 1 (POMT1))

Alternative Name: Pomt1

Background: Protein O-mannosyl-transferase 1 (EC 2.4.1.109) (Dolichyl-phosphate-mannose--protein mannosyltransferase 1),FUNCTION: Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient. Essentially dedicated to O-mannosylation of alpha-DAG1 and few other proteins but not of cadherins and protocaherins. {ECO:0000250|UniProtKB:Q9Y6A1}.

Molecular Weight: 85.2 kDa

UniProt: Q8R2R1

AlphaFold: Q8R2R1