CHAC1 Protein (AA 1-223) (His tag)

Catalog No. ABIN7559736

Recombinant CHAC1 protein expressed in HEK-293 Cells.

Quick Overview for CHAC1 Protein (AA 1-223) (His tag) (ABIN7559736)

Target: CHAC1 (ChaC, Cation Transport Regulator Homolog 1 (CHAC1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-223

Purification tag / Conjugate: This CHAC1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Chac1 Protein expressed in mammalian cells.

Sequence: MKQESASQST PPPSLSPAPS SAQPSWGDGD PQALWIFGYG SLVWKPDFAY SDSRVGFVRG YSRRFWQGDT FHRGSDKMPG RVVTLLEDRE GCTWGVAYQV RGEQVNEALK YLNVREAVLG GYDTKEVTFY PQDTPDQPLT ALAYVATPQN PGYLGPAPEE VIATQILACR GFSGHNLEYL LRLADFMQLC GPQAQDEHLE AIVDAVGTLL PCSYLPEQPL ALT Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: CHAC1 (ChaC, Cation Transport Regulator Homolog 1 (CHAC1))

Alternative Name: Chac1

Background: Glutathione-specific gamma-glutamylcyclotransferase 1 (Gamma-GCG 1) (EC 4.3.2.7) (Blocks Notch protein) (Botch) (Cation transport regulator-like protein 1),FUNCTION: Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade (By similarity). Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos (PubMed:22445366). {ECO:0000250|UniProtKB:Q9BUX1, ECO:0000269|PubMed:22445366, ECO:0000269|PubMed:23070364}.

Molecular Weight: 24.5 kDa

UniProt: Q8R3J5

AlphaFold: Q8R3J5