DAK Protein (AA 1-578) (His tag)

Catalog No. ABIN7559796

Recombinant DAK protein expressed in HEK-293 Cells.

Quick Overview for DAK Protein (AA 1-578) (His tag) (ABIN7559796)

Target: DAK (Dihydroxyacetone Kinase 2 Homolog (DAK))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-578

Purification tag / Conjugate: This DAK protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Tkfc Protein expressed in mammalian cells.

Sequence: MSSKKMVNSV EGCADDALAG LVASNPDLQL LQGHRVALRS DLDTLKGRVA LLSGGGSGHE PAHAGFIGKG MLTGVIAGSV FASPPVGSIL AAIRAVAQAG TVGTLLIVKN YTGDRLNFGL AMEQAKAEGI SVEMVIVEDD SAFTVLKKAG RRGLCGTVLI HKVAGALAEE GMGLEEITKR VSVIAKTMGT LGVSLSSCSV PGATHTFELA ADEIELGLGI HGEAGVRRIK IAPVDQIVTL MLDHMTNTSN IFHVPVRSGS SVVLIVNNLG GLSFLELGII ADAAIRLLEG RGVKVARALV GTFMSALEMP GVSLTLMLVD EPVLKLIDAE TTAKAWPHMA KVSVTGRKRI RAAPTEPPEA PEATAAGGVT SKQMALVLDR ICTTLIGLEE HLNALDRAAG DGDCGSTHSR AAKAIQGWLK EGPSLTSPAQ VLSRLSVLLL ERMGGSSGAL YGLFLTAAAQ PLKAKTDLPT WSAAMDAGLE SMQKYGKAAP GDRTMLDSLW AAAQEFQAWK SPGASLLPVL TKAVKSAEAA AEATKNMEAG AGRASYISSA QLDQPDPGAV AAAAIFRAIL EVLQTQGA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DAK (Dihydroxyacetone Kinase 2 Homolog (DAK))

Alternative Name: Tkfc

Background: Triokinase/FMN cyclase (Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)) [Includes: ATP-dependent dihydroxyacetone kinase (DHA kinase) (EC 2.7.1.28) (EC 2.7.1.29) (Glycerone kinase) (Triokinase) (Triose kinase), FAD-AMP lyase (cyclizing) (EC 4.6.1.15) (FAD-AMP lyase (cyclic FMN forming)) (FMN cyclase)],FUNCTION: Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Represses IFIH1-mediated cellular antiviral response. {ECO:0000250|UniProtKB:F1RKQ4, ECO:0000250|UniProtKB:Q3LXA3, ECO:0000250|UniProtKB:Q4KLZ6}.

Molecular Weight: 59.7 kDa

UniProt: Q8VC30

AlphaFold: Q8VC30