KPTN Protein (AA 1-430) (His tag)

Catalog No. ABIN7559837

Product Details

Target: KPTN (Kaptin (Actin Binding Protein) (KPTN))

Protein Type: Recombinant

Protein Characteristics: AA 1-430

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This KPTN protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Kptn Protein expressed in mammalian cells.

Sequence: MGEAAVAEGP CPLLEDSFTR FSSQSNVYGL AGGADGRGEL LAATLKGKVL GFRYQDLRQK IRPVAKELQF NYIPVDAEIV SIDTFNKSPP KRGLVVGITF IKDSGDKGSP FLNIYCDYEP GSEYNLDSIA ESCLNLELQF TPFQLCHAEV QVGDQLETVF LLSGNDPAIH LYKENEGLHQ FEEQPVENLF PELTNLTSSV LWLDVHNLPG SSQRLSALGC QSGYVRVAHV DQKNQEILQT WTIQQDGPIS RVIVFSLSAS EATQDSPQQE GYSLLVASML EPAVVYWDLL NKGLDDQLLL PGSDQFDSVL CGLVTDVDLD GQLEVLVATY GQELLCYKYR GLPEDSRGFR LLWRRSFASP LLAMAHVDLT GDGLRELAVI SLKGVHILQH SLIQASELVL TRLRHQVEQR KHQQGLGDRV GPRPVEHPAS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: KPTN (Kaptin (Actin Binding Protein) (KPTN))

Alternative Name: Kptn (KPTN Products)

Background: KICSTOR complex protein kaptin,FUNCTION: As part of the KICSTOR complex functions in the amino acid-sensing branch of the TORC1 signaling pathway. Recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes and allows its interaction with GATOR2 and the RAG GTPases. Functions upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in absence of amino acids. In absence of the KICSTOR complex mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also probably involved in the regulation of mTORC1 by glucose. {ECO:0000250|UniProtKB:Q9Y664}.

Molecular Weight: 47.5 kDa

UniProt: Q8VCX6

AlphaFold: Q8VCX6

Pathways: Sensory Perception of Sound