Calpain 8 Protein (CAPN8) (AA 1-703) (His tag)

Catalog No. ABIN7559981

This Recombinant Calpain 8 protein is expressed in HEK-293 Cells.

Quick Overview for Calpain 8 Protein (CAPN8) (AA 1-703) (His tag) (ABIN7559981)

Target: Calpain 8 (CAPN8)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-703

Purification tag / Conjugate: This Calpain 8 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Capn8 Protein expressed in mammalian cells.

Sequence: MAALAAGISK QRAAAQGLGS NQNAVKYLGQ DFETLRKQCL NSGVLFKDPE FPACPSALGY RDLGPGSAET QGIIWKRPTE LCSNPQFIVG GATRTDIRQG GLGDCWLLAA IASLTLNEKL LYRVVPRDQS FQKNYAGIFH FQFWQYGEWV EVVIDDRLPT KNGQLLFLHS EEGNEFWSAL LEKAYAKLNG SYEALAGGST IEGFEDFTGG ISEFYDLRKP PGNLYYTIQK ALRKGSLLGC SIDVSNAAEA EATTRQKLVK GHAYSVTGVE EVDFRGLPEK LIRLRNPWGE VEWTGAWSDS APEWNYIDPQ KKGELDKRAE DGEFWMSFSD FLKQFSRLEI CNLSPDSLSS EEIHKWNLVL FNGRWTRGST AGGCQNYPAT YWTNPQFKIH LDEVDEDQEE GTSEPCCTVL LGLMQKNRRR QRRIGQGMLS IGYAVYQIPK ELENHTDEHL GRDFFQGRQP STCSSTYMNL REVSSRVQLP PGQYLVVPST FEPFKDGDFC LRVFSEKKAQ ALEIGDAVPG DPHEPHPRDM DGEDEHFWSL SEEFADKDSE ISAHQLKRVL NGLLSKRTDM KFDGFNINTC REMISLLDGD GTGSLRPVEF KTLWLKICKY LEIYQEMDHS RAGTIDAHEM RTALKKAGFT LNNQVQQTIA TRYACSKLGV DFDGFVACMI RLEILFKLFR LLDKDQNGIV QLSLAEWLCR ALV Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Calpain 8 (CAPN8)

Alternative Name: Capn8

Background: Calpain-8 (EC 3.4.22.53) (New calpain 2) (nCL-2) (Stomach-specific M-type calpain),FUNCTION: Calcium-regulated non-lysosomal thiol-protease (By similarity). Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta-subunit of coatomer complex. {ECO:0000250, ECO:0000269|PubMed:16476741}.

Molecular Weight: 79.3 kDa

UniProt: Q91VA3

AlphaFold: Q91VA3