TRIM59 Protein (AA 1-403) (His tag)

Catalog No. ABIN7560244

Product Details

Target: TRIM59 (Tripartite Motif Containing 59 (TRIM59))

Protein Type: Recombinant

Protein Characteristics: AA 1-403

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This TRIM59 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Trim59 Protein expressed in mammalian cells.

Sequence: MHNFEEELTC PICYSIFEDP RVLPCSHTFC RNCLENVLQA SGNFYIWRPL RIPLKCPNCR SIIEIASTGI ESLPVNFALR AIIEKYQQED HPDVVTCPEH YRQPLNVYCL LDKKLVCGHC LTIGQHHGHP IDDLQSAYLK EKDTPQKLLK QLTDTHWTDI TRLIEKLEEQ KCHSEKIVQG DKEVVLQYFK ELIDTLEQKK KYFLAALCDV GKMINQEYTP QIQGMKEIRE QQLELMTITT SLQDESPLKF LEKIDEVRQR VQMLKQRPLP EVQPVEIYPR VSNVLKEEWS RIEIGRIKKA VIPEMRVSSK RTPCSWSDND EKEMELFKIL NIAIVSLISV ILMLILLFNH HIITFLNEIT SICFSEVFLS VYQSLSKNLY DLNNTVCYTL YLLKEFMWKI VSR Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TRIM59 (Tripartite Motif Containing 59 (TRIM59))

Alternative Name: Trim59 (TRIM59 Products)

Background: Tripartite motif-containing protein 59 (EC 2.3.2.27) (RING finger protein 1),FUNCTION: E3 ubiquitin ligase involved in different processes such as development and immune response (PubMed:22588174, PubMed:29467473). Serves as a negative regulator for innate immune signaling pathways by suppressing RLR-induced activation of IRF3/7 and NF-kappa-B via interaction with adapter ECSIT (PubMed:22588174). Regulates autophagy through modulating both the transcription and the ubiquitination of BECN1. On the one hand, regulates the transcription of BECN1 through negatively modulating the NF-kappa-B pathway. On the other hand, regulates TRAF6-mediated 'Lys-63'-linked ubiquitination of BECN1, thus affecting the formation of the BECN1-PIK3C3 complex. In addition, mediates 'Lys-48'-linked ubiquitination of TRAF6 and thereby promotes TRAF6 proteasomal degradation. Acts also as a critical regulator for early embryo development from blastocyst stage to gastrula through modulating F-actin assembly and WASH1 'Lys-63'-linked ubiquitination (PubMed:29467473). {ECO:0000250|UniProtKB:Q8IWR1, ECO:0000269|PubMed:22588174, ECO:0000269|PubMed:29467473}.

Molecular Weight: 47.2 kDa

UniProt: Q922Y2

AlphaFold: Q922Y2

Pathways: SARS-CoV-2 Protein Interactome