RNF112 Protein (AA 1-654) (His tag)

Catalog No. ABIN7560288

Recombinant RNF112 protein expressed in HEK-293 Cells.

Quick Overview for RNF112 Protein (AA 1-654) (His tag) (ABIN7560288)

Target: RNF112 (Ring Finger Protein 112 (RNF112))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-654

Purification tag / Conjugate: This RNF112 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Rnf112 Protein expressed in mammalian cells.

Sequence: MPRPVLSVTA FCHRLGKRES KRSFMGNSSN SWVLPREEAQ GWMGQAVQGG TRTSRSHASF PKLELGLGHR PSPTREPPTC SICLERLREP ISLDCGHDFC IRCFSTHRIP GCELPCCPEC RKICKQRKGL RSLGERMKLL PQRPLPPALQ ETCAVRAERL LLVRINASGG LILRMGAINR CLKHPLARDT PVCLLAVLGE QHSGKSFLLD HLLSGLPSLE SGDSGRPRAE GSLPGIRWGA NGLTRGIWMW SHPFLLGKEG KKVAVFLVDT GDVMSPELSK ETRVKLCALT MMLSSYQILN TSQELKDTDL GYLEMFVHVA EVMGKHYGMV PIQHLDLLVR DSSHHNKSGQ GHVGDILQKL SGKYPKVQEL LLGKRARCYL LPAPERQWVN KDQASPRGNT EDDFSHHFRA YILDVLSTAP QHAKSRCQGY WSEGRAVARG DRRLLTGQQL AQEIKNLSGW MGKTGPSFNS PDEMAAQLHD LRKVEAAKKE FEEYVRQQDI ATKRIFSALR VLPDTMRNLL STQKDAILAR HGVALLCKER EQTLEALEAE LQAEAKAFMD SYTMRFCGHL AAVGGAVGAG LMGLAGGVVG AGMAAAALAA EAGMVAAGAA VGATGAAVVG GGVGAGLAAT VGCMEKEEDE RVQGGDREPL LQEE Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RNF112 (Ring Finger Protein 112 (RNF112))

Alternative Name: Rnf112

Background: RING finger protein 112 (EC 2.3.2.27) (Brain finger protein) (Neurolastin) (Zinc finger protein 179),FUNCTION: E3 ubiquitin-protein ligase that plays an important role in neuronal differentiation, including neurogenesis and gliogenesis, during brain development. During embryonic development initiates neuronal differentiation by inducing cell cycle arrest at the G0/G1 phase through up-regulation of cell-cycle regulatory proteins (PubMed:21566658, PubMed:28684796). Plays a role not only in the fetal period during the development of the nervous system, but also in the adult brain, where it is involved in the maintenance of neural functions and protection of the nervous tissue cells from oxidative stress-induced damage (PubMed:27918959, PubMed:26792191, PubMed:26951452). Exhibits GTPase and E3 ubiquitin-protein ligase activities. Regulates dendritic spine density and synaptic neurotransmission, its ability to hydrolyze GTP is involved in the maintenance of dendritic spine density (PubMed:26212327). {ECO:0000269|PubMed:21566658, ECO:0000269|PubMed:26212327, ECO:0000269|PubMed:26792191, ECO:0000269|PubMed:26951452, ECO:0000269|PubMed:27918959, ECO:0000269|PubMed:28684796}.

Molecular Weight: 71.3 kDa

UniProt: Q96DY5

AlphaFold: Q96DY5