OOEP Protein (AA 1-164) (His tag)

Catalog No. ABIN7560661

This Recombinant OOEP protein is produced in HEK-293 Cells.

Quick Overview for OOEP Protein (AA 1-164) (His tag) (ABIN7560661)

Target: OOEP (Oocyte Expressed Protein (OOEP))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-164

Purification tag / Conjugate: This OOEP protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ooep Protein expressed in mammalian cells.

Sequence: MASHTADADA KPDSDSQKLL NVLPVSLRLR TRPWWFPIQE VSNPLVLYME AWVAERVIGT DQAEISEIEW MCQALLTVDS VNSGNLAEIT IFGQPSAQTR MKNILLNMAA WHKENELQRA VKVKEVEEFL KIRASSILSK LSKKGLKLAG FPLPLEGRET QMES Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: OOEP (Oocyte Expressed Protein (OOEP))

Alternative Name: Ooep

Background: Oocyte-expressed protein homolog (Factor located in oocytes permitting embryonic development) (Floped) (Oocyte- and embryo-specific protein 19) (mOEP19) (STAT3 downstream gene and differentiation regulator),FUNCTION: As part of the OOEP-KHDC3 scaffold, recruits BLM and TRIM25 to DNA replication forks, thereby promoting the ubiquitination of BLM by TRIM25, enhancing BLM retainment at replication forks and therefore promoting stalled replication fork restart (PubMed:29125140). Positively regulates the homologous recombination-mediated DNA double-strand break (DSB) repair pathway by regulating ATM activation and RAD51 recruitment to DSBs in oocytes (PubMed:29955025). Thereby contributes to oocyte survival and the resumption and completion of meiosis (PubMed:29955025). As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:18804437, PubMed:25208553). Required for the formation of F-actin cytoplasmic lattices in oocytes which in turn are responsible for symmetric division of zygotes via the regulation of mitotic spindle formation and positioning (PubMed:25208553). {ECO:0000269|PubMed:18804437, ECO:0000269|PubMed:25208553, ECO:0000269|PubMed:29125140, ECO:0000269|PubMed:29955025}.

Molecular Weight: 18.5 kDa

UniProt: Q9CWE6

AlphaFold: Q9CWE6