Diphthamide Biosynthesis 7 (DPH7) (AA 1-477) protein (His tag)

Catalog No. ABIN7560760

This Recombinant Diphthamide Biosynthesis 7 protein is produced in HEK-293 Cells.

Quick Overview for Diphthamide Biosynthesis 7 (DPH7) (AA 1-477) protein (His tag) (ABIN7560760)

Target: Diphthamide Biosynthesis 7 (DPH7)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-477

Purification tag / Conjugate: His tag

Purpose: Predefined custom protein recombinant Dph7 Protein expressed in mammalian cells.

Sequence: MAGSHAGTLR VLQAVDTELT ADSVEWCPVE GYQHLLACGT YQLRAPRDQP ALDGSEPQVR LGRLYLFSFS EHNTAKPLLE VQRRDSSAVL DMKWCHIPVS GHVLLGLANA SGSIGLLRLM ECENNSYTLQ PISSLALDEN CLSLSMDWST GKSVRAREQP LKIISSDSKG QLHLLMVNEG TAELQLVASW PAHHFEAWIA AFNYWQTELV YSGGDDCLLR GWDTRMLGTP VFTSKRHCMG VCSIQSSPHQ EHILATGSYD EHVLLWDTRN IRQPLADVPV QGGVWRLKWH PVHHHLLLAA CMHNGFKILN CQKAIEEKQD ITVLTSHEMP NSLVYGADWS WLFHSMKPTP TWFFDQNDMG VKAADHSSLK VTEEPPIHSQ EQTMDRQVEG PANAHTRAEL KASLLPLTED MKNSKDCSSS SVKTRDLSHC SGGQSFDNSL LATCSFYDHV LHLWKWETNQ ARTLCSGTGC DLGSADH Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Diphthamide Biosynthesis 7 (DPH7)

Alternative Name: Dph7

Background: Diphthine methyltransferase (EC 3.1.1.97) (Diphthamide biosynthesis protein 7) (DPH7) (WD repeat-containing protein 85),FUNCTION: Catalyzes the demethylation of diphthine methyl ester to form diphthine, an intermediate diphthamide biosynthesis, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). {ECO:0000250|UniProtKB:Q9BTV6}.

Molecular Weight: 53.2 kDa

UniProt: Q9CYU6

AlphaFold: Q9CYU6