RMI1 Protein (AA 1-616) (His tag)

Catalog No. ABIN7560989

This Recombinant RMI1 protein is expressed in HEK-293 Cells.

Quick Overview for RMI1 Protein (AA 1-616) (His tag) (ABIN7560989)

Target: RMI1 (Homolog of Yeast RecQ-mediated Genome Instability 1 (RMI1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-616

Purification tag / Conjugate: This RMI1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Rmi1 Protein expressed in mammalian cells.

Sequence: MSVASAVLRV ETWLLATWHV KVPPMWLEAC VNWIQEENNN ATLSQAQINK QVLEQWLLTD LRDLEHPLLP DDILELPKGE LNGFYALQIN SLVDVSQPAY SQIQKLRGKN TTNDLVSAET QSTPKPWEVR PSRMLMLQLT DGVTHIQGME YQSIPALHSG LPPGTKILVR GCILFRLGVL LLKPENVKVL GGEVDGLSEE NAQEKVLARL IGELDPTVPV IPNNSIHNVP KVSGGLDAVL GPSDEELLAS LDESEESAAN NDVAMERSCF STGTSSNTTP TNPSGFEPGC NISSRPKEKP PNQPTHFTDG EFDDFSLEEA LLLEETVQKE QMETKASQPL TLKENTGKCM EIFSHKPSSL NHTALIHKQG NSNFDEKTSE QMIHEDKFFD CASTRNHHKR FSAHDFTNDS KISEVDDAAQ QTLSSSNVHC LRNKILNRKL DLSEKSSQIS KENGHPFQAC SSRSFENNTY LSIGMDLHSP PFIYLSVLMA RKPKEVTTVT VKAFIVTLTG NLSSSGGFWG VTAKVSDGTA YLDVDFIDEI LTSMIGYSVP EMKQLRKDPL KYKTFLEGLQ KCQRDLIDLC CLMTISYDPS SCKGVVLELQ DVGMEHVENL KKRLNK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RMI1 (Homolog of Yeast RecQ-mediated Genome Instability 1 (RMI1))

Alternative Name: Rmi1

Background: RecQ-mediated genome instability protein 1,FUNCTION: Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability (By similarity). {ECO:0000250}.

Molecular Weight: 68.5 kDa

UniProt: Q9D4G9

AlphaFold: Q9D4G9

Pathways: DNA Damage Repair