PRPF4 Protein (AA 1-521) (His tag)

Catalog No. ABIN7561245

Recombinant PRPF4 protein expressed in HEK-293 Cells.

Quick Overview for PRPF4 Protein (AA 1-521) (His tag) (ABIN7561245)

Target: PRPF4 (PRP4 Pre-mRNA Processing Factor 4 Homolog (PRPF4))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-521

Purification tag / Conjugate: This PRPF4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Prpf4 Protein expressed in mammalian cells.

Sequence: MASSRASSTT TKTKAPDDLV APVVKKPHIY YGSLEEKERE RLAKGESGIL GKEGLKAGIE AGNINITSGE VFEIEEHISE RQAEVLAEFE RRKRARQINV STDDSEVKAC LRALGEPITL FGEGPAERRE RLRNILSVVG TDALKKTKKD DEKSKKSKEE YQQTWYHEGP NSLKVARLWI ANYSLPRAMK RLEEARLHKE IPETTRTSQM QELHKSLRSL NNFCSQIGDD RPISYCHFSP NSKMLATACW SGLCKLWSVP DCSLLHTLRG HNTNVGAIVF HPKSTVSLDQ KDVNLASCAA DGSVKLWSLD SDEPVADIEG HTVRVARVMW HPSGRFLGTT CYDRSWRLWD LEAQEEILHQ EGHSMGVYDI AFHQDGSLAG TGGLDAFGRV WDLRTGRCIM FLEGHLKEIY GINFSPNGYH IATGSGDNTC KVWDLRQRRC VYTIPAHQNL VTGVKFEPIH GDFLLTGAYD NTAKIWTHPG WSPLKTLAGH EGKVMGLDIS SDGQLIATCS YDRTFKLWMA E Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PRPF4 (PRP4 Pre-mRNA Processing Factor 4 Homolog (PRPF4))

Alternative Name: Prpf4

Background: U4/U6 small nuclear ribonucleoprotein Prp4 (U4/U6 snRNP 60 kDa protein) (WD splicing factor Prp4),FUNCTION: Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). {ECO:0000250|UniProtKB:O43172}.

Molecular Weight: 58.4 kDa

UniProt: Q9DAW6

AlphaFold: Q9DAW6