GTF2H2 Protein (AA 1-396) (His tag)

Catalog No. ABIN7561542

Recombinant GTF2H2 protein expressed in HEK-293 Cells.

Quick Overview for GTF2H2 Protein (AA 1-396) (His tag) (ABIN7561542)

Target: GTF2H2 (General Transcription Factor IIH, Polypeptide 2, 44kDa (GTF2H2))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-396

Purification tag / Conjugate: This GTF2H2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Gtf2h2 Protein expressed in mammalian cells.

Sequence: MDEEPERTKR WEGGYERTWE ILKEDETGSL KATIEDILFK AKRKRVFEHH GQVRLGMMRH LYVVVDGSRT MEDQDLKPNR LTCTLKLLEY FVEEYFDQNP ISQIGIIVTK SKRAEKLTEL SGNPRKHITS LKKAVDMTCH GEPSLYNSLS MAMQTLKHMP GHTSREVLII FSSLTTCDPS NIYDLIKTLK TAKIRVSVIG LSAEVRVCTV LARETGGTYH VILDETHYKE LLAHHVSPPP ASSSSECSLI RMGFPQHTIA SLSDQDAKPS FSMAHLDNNS TEPGLTLGGY FCPQCRAKYC ELPVECKICG LTLVSAPHLA RSYHHLFPLD AFQEISLEEY KGERFCYGCQ GELKDQHVYV CTVCQNVFCV DCDVFVHDSL HCCPGCIHKI PTPSGI Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: GTF2H2 (General Transcription Factor IIH, Polypeptide 2, 44kDa (GTF2H2))

Alternative Name: Gtf2h2

Background: General transcription factor IIH subunit 2 (Basic transcription factor 2 44 kDa subunit) (BTF2 p44) (General transcription factor IIH polypeptide 2) (TFIIH basal transcription factor complex p44 subunit),FUNCTION: Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. The N-terminus of GTF2H2 interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter. {ECO:0000250|UniProtKB:Q13888}.

Molecular Weight: 44.7 kDa

UniProt: Q9JIB4

AlphaFold: Q9JIB4

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling