DBF4 Protein (AA 1-663) (His tag)

Catalog No. ABIN7561814

Product Details

Target: DBF4 (DBF4 Homolog (DBF4))

Protein Type: Recombinant

Protein Characteristics: AA 1-663

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This DBF4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Dbf4 Protein expressed in mammalian cells.

Sequence: MNLETMRIHS KAPLPGGIQD RNEKNRPSLK SLKADNRLEK SKYKPLWGKI FYLDLPSITI CEKLQKDIKE LGGRVEEFLS KDISYFVSNK KEAKYAQTLG RVSPVPSPES AYTAETTSPH PSHDGSSFKS QDRVCLSRGK LLAEKAVKDH DFIPANSILS NALSWGVKIL HIDDIRYYIE QKKKALSALK KSSASGKDAG KKAGPGIQKT RTGRLKKPFL KVEDVNRCYR PFYLQLPSLP CINYFLQKPC SPFDIEKSSS VQKQAQPKLR INMDGDKCGT PVQLQLKEKR KKGYCECCLQ KYEDLETHLL SEKHRNFAQS NQYQVVDDIV SQLVFDFVEY GRDTPQKKRI RYSVGSLSSV SANVLKNTAP KEKPLLEPNF QKDVGESSGH LLKPNSQYEE TQKPEEKHGF ASEPTTYSSA GLKGCDRKPV SMFNASEPDP EQEYAQLPLR DSTPEHQVTE GRNDGEQRVD PAPGVSQSCG QVSHLSTESN LPQPQLAADI TQLSAKDLQE KGFHVVIGHA SDLVALNTSK EQLTMKARTP PCSPQEPHEC DTENMENLPC GKIQRKVRML LGQQKANAEP SAELDKKRTE YLPAHEDRTC GSPVQSLLDL FQTSEEKSEF LGFTGYTENS GICDVLDIWE EENSSTLLST FFSSPSTSAF VGF Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DBF4 (DBF4 Homolog (DBF4))

Alternative Name: Dbf4 (DBF4 Products)

Background: Protein DBF4 homolog A (MuDBF4),FUNCTION: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role DNA in replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle. {ECO:0000269|PubMed:10517317}.

Molecular Weight: 74.2 kDa

UniProt: Q9QZ41

AlphaFold: Q9QZ41

Pathways: Mitotic G1-G1/S Phases, DNA Replication