MBD5 Protein (AA 1-1498) (His tag)

Catalog No. ABIN7562039

This Recombinant MBD5 protein is expressed in HEK-293 Cells.

Quick Overview for MBD5 Protein (AA 1-1498) (His tag) (ABIN7562039)

Target: MBD5 (Methyl-CpG Binding Domain Protein 5 (MBD5))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1498

Purification tag / Conjugate: This MBD5 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Mbd5 Protein expressed in mammalian cells.

Sequence: MNGGKECDGG DKEGGLAAIQ VPVGWQRRVD HNGVLYISPS GSLLSCLDQV KTYLLTDGTC KCGLECPLIL PKVFNFDPGA AVKQRTAEDV KADDDVTKLC IHKRKIIAVA TLHQSMEAPH PSLVLTSPGG GTNATPVVPS RAATPRSVRN KSHEGITNSV MPECKNPFKL MTGSSNAMGR LYMQDLPGSQ QQELHPVYPR QRLGSSEHGQ KSPFRGSHGG LPSPASSGSQ IYGDGSISPR TDPLGSPDVF TRNNPGFHGA PNSSPIHLNR TPLSPPSVML HGSPVQSSCA MAGRTNIPLS PTLTTKSPVM KKPMCNFSTN MEIPRAMFHH KPPQGPPPPP PPSCALQKKP LTSEKDPLGI LDPIPSKPVN QNPIIINPTS FHSNVHSQVP VMNVSMPPAV VPLPSNLPLP TVKPGHMNHG SHVQRIQHSA STSLSPSPVT SPVHMMGTGI GRIEASPQRS RSSSTSSDHG NFMMPPVGPQ ATCSGIKVPP RSPRSTIGSP RPSMPSSPST KSDGHHQYKD IPNPLIAGMS NVLNTPSSAA FPTAPAGNGS VKSQPGLLGM PLNQILNQHN AASFPASSLL SAAAKAQLAN QNKLAGNNSS SSNNSGAVAS SGNTEGHSTL NTMFPPTANM LLPTGEGQSG RAALRDKLMS QQKDSLRKRK QPPTTVLSLL RQSQMDSSAA PKPGPDLLRK HGQGSFPISS MSQLLQSMSC QSSHLSSNST PGCGGSNTAL PCSANQLHFP DPNMNSTVLQ NSLTQSIPLR GEAVHCHNAN TNFVHSNSPV PNHHLAGLIN QIQASGNCGM LSQSGMALGN SLHPNPPQSR ISTSSTPVIP NSIVSSYNQT SSEAGGSSLP SSIAIAGSNH PAITKTTSVL QDGVIVTTAA GNPLQSQLPI GSDFPFVGQE HALHFPSNST ANNHLPHPLN PSLLSSLPIS LPVNQQHLLN QNLLNILQPS AGEGDISSIN NSLNNHQLTH LQSLLNSNQM FPPNQQPQQH LLQGHQNLQA FQGQPTVPCP ANNNPMACLF QNFQVRMQGD AALLNKRIST QPGLTTLPEN PNLALPHFQD TPCELQPRID LGQPMKDGLV MGGQGDASVD AIYKAVVDAA SKGMQVVITT AVNSTTQISP IPALSAMSAF TASIGDPLNL SSAVSAVIHG RNMGGVDHDG RLRNARGARL PKNIDHGKNS SEGDGFECFK SASCHTSRKQ WDGEQSPRGE RNRWKYEEFL DHPGHIHSSP CHERPNNVST LPFLAGEQHP ILLPPRNCQG DKILEENFRY NNYKRTMMSF KERLESTVER CTHINGNRPR QSRGFGELLG TAKQDLVLEG QSPGSSNSLE SSLVKDYIHY NGDFNAKTIN GCVPSPSDAK SISSEDDLRN PDSPSSHELI HYRPRTFNVG DLVWGQIKGL TSWPGKFIRE DDVHNSCQQS PEEGKVEPEK LKTLTEGLEA YSRVRKRSRK SGKLNNHLEA AIHEAMSELD KMSGTVHQIP QGDRQMRPPK PKRRKISR Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MBD5 (Methyl-CpG Binding Domain Protein 5 (MBD5))

Alternative Name: Mbd5

Background: Methyl-CpG-binding domain protein 5 (Methyl-CpG-binding protein MBD5),FUNCTION: Binds to heterochromatin. Does not interact with either methylated or unmethylated DNA (in vitro) (By similarity). {ECO:0000250}.

Molecular Weight: 160.3 kDa

UniProt: B1AYB6

AlphaFold: B1AYB6

Pathways: Chromatin Binding