ABCB5 Protein (AA 1-1255) (His tag)

Catalog No. ABIN7562069

This Recombinant ABCB5 protein is expressed in HEK-293 Cells.

Quick Overview for ABCB5 Protein (AA 1-1255) (His tag) (ABIN7562069)

Target: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1255

Purification tag / Conjugate: This ABCB5 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Abcb5 Protein expressed in mammalian cells.

Sequence: MANSERTNGL QETNQRYGPL QEQVPKVGNQ AVGPIEIFRF ADNLDIVLMT LGILASMING ATVPLMSLVL GEISDHLING CLVQTNRTKY QNCSQTQEKL NEDIIVLTLY YIGIGAAALI FGYVQISFWV ITAARQTTRI RKQFFHSILA QDISWFDGSD ICELNTRMTG DINKLCDGIG DKIPLMFQNI SGFSIGLVIS LIKSWKLSLV VLSTSPLIMA SSALCSRMII SLTSKELDAY SKAGAVAEEA LSSIQTVTAF GAQEKEIQRY TQHLKDAKDA GIKRATASKL SLGAVYFFMN GAYGLAFWYG TSLIFGGEPG YTIGTILAVF FSVIHSSYCI GSVAPHLETF TVARGAAFNI FQVIDKKPNI DNFSTAGFVP ECIEGNIEFK NVSFSYPSRP SAKVLKGLNL KIKAGETVAL VGPSGSGKST TVQLLQRLYD PEDGCITVDE NDIRAQNVRH YREQIGVVRQ EPVLFGTTIG NNIKFGREGV GEKEMEQAAR EANAYDFIMA FPKKFNTLVG EKGAQMSGGQ KQRIAIARAL VRNPKILILD EATSALDTES ESLVQTALEK ASKGRTTIVV AHRLSTIRGA DLIVTMKDGM VVEKGTHAEL MAKQGLYYSL AMAQDIKKVD EQMESRTCST AGNASYGSLC DVNSAKAPCT DQLEEAVHHQ KTSLPEVSLL KIFKLSKSEW PFVVLGTLAS ALNGSVHPVF SIIFGKLVTM FEDKNKATLK QDAELYSMML VVLGIVALVT YLMQGLFYGR AEENLAMRLR HSAFKAMLYQ DMAWYDDKEN NTGALTTTLA VDVAQIQGAA TSRLGIVTQD VSNMSLSILI SFIYGWEMTL LILSFAPVLA VTGMIQTAAM AGFANRDKQA LKRAGKIATE AVENIRTVVS LTRERAFEQM YEETLQTQHR NALKRAHITG CCYAVSHAFV HFAHAAGFRF GAYLIQAGRM MPEGMFIVFT AIAYGAMAIG ETLVWAPEYS KAKAGASHLF ALLKNKPTIN SCSQSGEKPD TCEGNLEFRE VSFVYPCRPE VPVLQNMSLS IEKGKTVAFV GSSGCGKSTC VQLLQRFYDP MKGQVLLDGV DVKELNVQWL RSQTAIVSQE PVLFNCSIAE NIAYGDNSRM VPLEEIKEVA DAANIHSFIE GLPRKYNTLV GLRGVQLSGG QKQRLAIARA LLRKPKILLL DEATSALDNE SEKVVQQALD KARRGKTCLV VAHRLSTIQN ADMIVVLQNG SIKEQGTHQE LLRNGDTYFK LVAAH Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Alternative Name: Abcb5

Background: ATP-binding cassette sub-family B member 5 (ABCB5 P-gp) (P-glycoprotein ABCB5) (EC 7.6.2.2),FUNCTION: Energy-dependent efflux transporter responsible for decreased drug accumulation in multidrug-resistant cells (By similarity). Specifically present in limbal stem cells, where it plays a key role in corneal development and repair (PubMed:25030174). {ECO:0000250|UniProtKB:Q2M3G0, ECO:0000269|PubMed:25030174}.

Molecular Weight: 137.4 kDa

UniProt: B5X0E4

AlphaFold: B5X0E4