AKAP5 Protein (AA 1-745) (His tag)

Catalog No. ABIN7562089

Product Details

Target: AKAP5 (A Kinase (PRKA) Anchor Protein 5 (AKAP5))

Protein Type: Recombinant

Protein Characteristics: AA 1-745

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This AKAP5 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Akap5 Protein expressed in mammalian cells.

Sequence: METSVSEIQV ETKDEKGPVA ASPQKERQER KTATLCFKRR KKANKTKPKA GSRTAEETKK HTPEAGGSGQ RQPAGAWASI KGLVTHRKRS EPAKKQKPPE AEVQPEDGAL PKKKAKSRLK FPCLRFSRGA KRSRHSKLTE DSGYVRVQGE ADDLEIKAQT QPDDQAIQAG STQGLQEGVL VRDGKKSQES HISNSVTSGE NVIAIELELE NKSSAIQMGT PELEKETKVI TEKPSVQTQR ASLLESSAAG SPRSVTSAAP PSPATTHQHS LEEPSNGIRE SAPSGKDDRR KTAAEEKKSG ETALGQAEEA AVGQADKRAL SQAGEATAGH PEEATVIQAE SQAKEGKLSQ AEETTVAQAK ETVLSQAKEG ELSQAKKATV GQAEEATIDH TEKVTVDQAE ETTVGQAEEA TVGQAGEAIL SQAKEATVVG QAEEATVDRA EEATVGQAEE ATVGHTEKVT VDQAEEATVG QAEEATVGQA EEATVDWAEK PTVGQAEEAT VGQAEEATVG HTEKVTVDQA EEATVGQAEE ATVGHTEKVT VDHAEEATVG QAEEATVGQA EKVTVDHAEE ATVGQAEEAT VGQAEKVTVD HAEEATVGQA EEATVGQAEK VTVDQAEEPT VDQAEEAISS HAPDLKENGI DTEKPRSEES KRMEPIAIII TDTEISEFDV KKSKNVPKQF LISMENEQVG VFANDSDFEG RTSEQYETLL IETASSLVKN AIELSVEQLV NEMVSEDNQI NTLFQ Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: AKAP5 (A Kinase (PRKA) Anchor Protein 5 (AKAP5))

Alternative Name: Akap5 (AKAP5 Products)

Target Type: Viral Protein

Background: A-kinase anchor protein 5 (AKAP-5) (A-kinase anchor protein 150 kDa) (AKAP 150) (P150) (cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein),FUNCTION: Multivalent scaffold protein that anchors the cAMP-dependent protein kinase/PKA to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade. Plays a role in long term synaptic potentiation by regulating protein trafficking from the dendritic recycling endosomes to the plasma membrane and controlling both structural and functional plasticity at excitatory synapses. {ECO:0000250|UniProtKB:P24588}.

Molecular Weight: 79.4 kDa

UniProt: D3YVF0

AlphaFold: D3YVF0

Pathways: cAMP Metabolic Process