TRF2 Protein (AA 1-541) (His tag)

Catalog No. ABIN7562271

Product Details

Target: TRF2 (TERF2) (Telomeric Repeat Binding Factor 2 (TERF2))

Protein Type: Recombinant

Protein Characteristics: AA 1-541

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This TRF2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Terf2 Protein expressed in mammalian cells.

Sequence: MAAGAGTAGP ASGPGVVRDP MASQPRKRPS REGGEGGEGE RRSNTMAGGG GSSDSSGRAA SRRASRSGGR ARRGRHEPGL GGAAERGAGE ARLEEAVNRW VLKFYFHEAL RAFRSSRYRD FRQIRDIMQA LLVRPLGKEH TVSRLLRVMQ CLSRIEEGEN LDCSFDMEAE LTPLESAINV LEMIKTEFTL TDSMVESSRK LVKEAAVIIC IKNKEFEKAS KILKKYMSKD PTTQKLRTDL LNIIREKNLA HPVIQNFSYE VFQQKMLRFL ESHLDDTEPY LLTMAKKALK SESAASSTMR EEKHPEPVEK PLREPPSRQP QNPPATIGIR TLKAAFKALS TAQDSEAAFA KLDQKDLVLA NLASPSSPAH KHKRPRKDEH ESAAPAEGEG GSDRQPRNSP MTISRLLLEE DSQSTEPSPG LNSSHKAMSA SKPRALNQPH PGEKKPKASK DKWNSPNGLE EKEVWLEEDQ LFEVQAPGED RSSSLTRKQK WTIEESEWVK DGVRKYGEGN WAAISKSYPF VNRTAVMIKD RWRTMKKLGM N Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TRF2 (TERF2) (Telomeric Repeat Binding Factor 2 (TERF2))

Alternative Name: Terf2 (TERF2 Products)

Background: Telomeric repeat-binding factor 2 (TTAGGG repeat-binding factor 2) (Telomeric DNA-binding protein),FUNCTION: Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends, without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length. {ECO:0000269|PubMed:20339076, ECO:0000269|PubMed:20619712, ECO:0000269|PubMed:20622870}.

Molecular Weight: 60.3 kDa

UniProt: O35144

AlphaFold: O35144

Pathways: Cell Division Cycle, Telomere Maintenance