TOP3A Protein (AA 1-1003) (His tag)

Catalog No. ABIN7562511

Product Details

Target: TOP3A (Topoisomerase (DNA) III alpha (TOP3A))

Protein Type: Recombinant

Protein Characteristics: AA 1-1003

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This TOP3A protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Top3a Protein expressed in mammalian cells.

Sequence: MIFPVTLLAF QWHRRPGGRA LSRAAMEVAF RGVRKVLCVA EKNDAAKGIA DLLSNGRMRR KEGLSKFNKI YEFDYHLYGQ NVTMIMTSVS GHLLAHDFQM QFRKWQSCNP LVLFEAEIEK YCPENFIDIK KTLERETHHC QALVIWTDCD REGENIGFEI IHVCKAVKPN LRVLRARFSE ITPHAVRTAC ENLTEPDQRV SDAVDVRQEL DLRIGAAFTR FQTLRLQRIF PEVLAEQLIS YGSCQFPTLG FVVERFKAIQ AFVPEVFHKI KVTHDHKDGT VEFNWKRYRL FNHTACLVLY QLCMEDPMAT VVEVRSKPKS KWRPQALDTV ELEKLASRKL RINAKETMRI AEKLYTQGYI SYPRTETNIF PKDLNLVALV EQQTVDPHWG AFAQTILERG GPTPRNGSKS DQAHPPIHPT KYTSGLQGDD RRLYEFIVRH FLACCSQDAQ GQETTVEIDI AQERFVAHGL IILARNYLDV YPYDHWSDKL LPVYEQGSHF QPSTVEMVDG ETSPPQLLTE ADLIALMEKH GIGTDATHAE HIETIKARMY VGLTSDKRFL PGHLGMGLVE GYDSMGYEMS KPDLRAELEA DLKLICEGKK DKFQVLRQQV QKYKQVFIEA VAKAKKLDEA LSQYLGERTE MAQQEEIYPA MPEPVRKCPQ CNKDMVLKTK KSGGFYLSCM GFPECRSAVW FPDSVLEASR DNSVCSVCQP PPVYRLKLKF KRGSLPPAMP LEFVGCIGGC DETLKEIFGL RFPRALPRAS QPSGHLQASQ ALNRMDSSQH NLSQPLVNRH TRPSKTVAQA LLPPTTAGES NSVTCNCGRE AVLLTVRKQG PNQGRHFYKC SNGDCNFFLW ADSSHSTGGG TPTSASGPPG SSVGCPSSVG SHMDGFGSLG SDSDGGTPCL CGQPAVTRTV QKDGPNKGRQ FHTCAKPREQ QCGFFQWVDE NVAPGSFAAP AWPGGRGKAQ RPEAASKRPR AGSSDAGSTV KKPRKCSLCH QPGHTRTFCP QNR Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TOP3A (Topoisomerase (DNA) III alpha (TOP3A))

Alternative Name: Top3a (TOP3A Products)

Background: DNA topoisomerase 3-alpha (EC 5.6.2.1) (DNA topoisomerase III alpha),FUNCTION: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. As an essential component of the RMI complex it is involved in chromosome separation and the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity. It is required for mtDNA decatenation and segregation after completion of replication, in a process that does not require BLM, RMI1 and RMI2. {ECO:0000250|UniProtKB:Q13472}.

Molecular Weight: 112.4 kDa

UniProt: O70157

AlphaFold: O70157

Pathways: DNA Damage Repair