Pol (AA 1-1281) protein (His tag)

Catalog No. ABIN7562835

Recombinant Pol protein expressed in HEK-293 Cells.

Quick Overview for Pol (AA 1-1281) protein (His tag) (ABIN7562835)

Target: Pol

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1281

Purification tag / Conjugate: His tag

Purpose: Predefined custom protein recombinant Pol Protein expressed in mammalian cells.

Sequence: MPTLTTKIKG SNNYFSLISL NINGLNSPIK RHRLTDWLHK QDPTFCCLQE THLREKDRHY LRVKGWKTIF QANGLKKQAG VAILISDKID FQPKVIKKDK EGHFILIKGK ILQEELSILN IYAPNARAAT FIRDTLVKLK AYIAPHTIIV GDFNTPLSSK DRSWKQKLNR DTVKLTEVMK QMDLTDIYRT FYPKTKGYTF FSAPHGTFSK IDHIIGHKTG LNRYKNIEIV PCILSDHHGL RLIFNNNINN GKPTFTWKLN NTLLNDTLVK EGIKKEIKDF LEFNENEATT YPNLWDTMKA FLRGKLIALS ASKKKRETAH TSSLTTHLKA LEKKEANSPK RSRRQEIIKL RGEINQVETR RTIQRINQTR SWFFEKINKI DKPLARLTKG HRDKILINKI RNEKGDITTD PEEIQNTIRS FYKRLYSTKL ENLDEMDKFL DRYQVPKLNQ DQVDHLNSPI SPKEIEAVIN SLPTKKSPGP DGFSAEFYQT FKEDLIPILH KLFHKIEVEG TLPNSFYEAT ITLIPKPQKD PTKIENFRPI SLMNIDAKIL NKILANRIQE HIKAIIHPDQ VGFIPGMQGW FNIRKSINVI HYINKLKDKN HMIISLDAEK AFDKIQHPFM IKVLERSGIQ GPYLNMIKAI YSKPVANIKV NGEKLEAIPL KSGTRQGCPL SPYLFNIVLE VLARAIRQQK EIKGIQIGKE EVKISLLADD MIVYISDPKN STRELLNLIN SFGEVVGYKI NSNKSMAFLY TKNKQAEKEI RETTPFSIVT NNIKYLGVTL TKEVKDLYDK NFKSLKKEIK EDLRRWKDLP CSWIGRINIV KMAILPKAIY RFNAIPIKIP TQFFNELEGA ICKFVWNNKK PRIAKSLLKD KRTSGGITMP DLKLYYRAIV IKTAWYWYRD RQVDQWNRIE DPEMNPHTYG HLIFDKGAKT IQWKKDSIFN NWCWHNWLLS CRRMRIDPYL SPCTKVKSKW IKELHIKPET LKLIEEKVGK SLEDMGTGEK FLNRTAMACA VRSRIDKWDL MKLQSFCKAK DTVNKTKRPP TDWERIFTYP KSDRGLISNI YKELKKVDFR KSNNPIKKWG SELNKEFSPE EYRMAEKHLK KCSTSLIIRE MQIKTTLRFH LTPVRMAKIK NSGDSRCWRG CGERGTLLHC WWECRLVQPL WKSVWRFLRK LDIVLPEDPA IPLLGIYPED APTGKKDTCS TMFIAALFII ARSWKEPRCP STEEWIQKMW YIYTMEYYSA IKKNEFMKFL AKWMDLEGII LSEVTHSQRN SHNMYSLISG Y Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Pol

Target Type: Viral Protein

Background: LINE-1 retrotransposable element ORF2 protein (ORF2p) (Long interspersed element-1) (L1) (Retrovirus-related Pol polyprotein LINE-1) [Includes: Reverse transcriptase (EC 2.7.7.49), Endonuclease (EC 3.1.21.-)],FUNCTION: Has reverse transcriptase activity required for target-primed reverse transcription of the LINE-1 element mRNA, a crucial step in LINE-1 retrotransposition. Also has endonuclease activity that allows the introduction of nicks in the chromosomal target DNA. Cleaves DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to sites of LINE-1 integration in the genome. Conformational properties of the target DNA sequence rather than specific nucleotides are key determinants of the ORF2p capacity for sequence-specific DNA recognition. Does not bend the DNA helix unlike related endonucleases but causes compression near the cleavage site. {ECO:0000250|UniProtKB:O00370}.

Molecular Weight: 149.6 kDa

UniProt: P11369

AlphaFold: P11369