GADD34 Protein (AA 1-657) (His tag)

Catalog No. ABIN7562916

Recombinant GADD34 protein expressed in HEK-293 Cells.

Quick Overview for GADD34 Protein (AA 1-657) (His tag) (ABIN7562916)

Target: GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-657

Purification tag / Conjugate: This GADD34 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ppp1r15a Protein expressed in mammalian cells.

Sequence: MAPSPRPQHV LHWRDAHNFY LLSPLMGLLS RAWSRLRGPE VPEAWLAKTV TGADQIEAAA LLTPTPVSGN LLPHGETEES GSPEQSQAAQ RLCLVEAESS PPETWGLSNV DEYNAKPGQD DLREKEMERT AGKATLQPAG LQGADKRLGE VVAREEGVAE PAYPTSQLEG GPAENEEDGE TVKTYQASAA SIAPGYKPST PVPFLGEAEH QATEEKGTEN KADPSNSPSS GSHSRAWEYY SREKPKQEGE AKVEAHRAGQ GHPCRNAEAE EGGPETTFVC TGNAFLKAWV YRPGEDTEEE DNSDSDSAEE DTAQTGATPH TSAFLKAWVY RPGEDTEEED SDSDSAEEDT AQTGATPHTS AFLKAWVYRP GEDTEEENSD LDSAEEDTAQ TGATPHTSAF LKAWVYRPGE DTEEENSDLD SAEEDTAQTG ATPHTSPFLK AWVYRPGEDT EDDTEEEEDS ENVAPGDSET ADSSQSPCLQ PQRCLPGEKT KGRGEEPPLF QVAFYLPGEK PESPWAAPKL PLRLQRRLRL FKAPTRDQDP EIPLKARKVH FAEKVTVHFL AVWAGPAQAA RRGPWEQFAR DRSRFARRIA QAEEKLGPYL TPDSRARAWA RLRNPSLPQS EPRSSSEATP LTQDVTTPSP LPSETPSPSL YLGGRRG Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))

Alternative Name: Ppp1r15a

Background: Protein phosphatase 1 regulatory subunit 15A (Growth arrest and DNA damage-inducible protein GADD34) (Myeloid differentiation primary response protein MyD116),FUNCTION: Recruits the serine/threonine-protein phosphatase PPP1CA to prevents excessive phosphorylation of the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress (PubMed:11381086, PubMed:12824288). Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1 (By similarity). May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15' (By similarity). Plays an essential role in autophagy by tuning translation during starvation, thus enabling lysosomal biogenesis and a sustained autophagic flux (By similarity). Acts also a viral restriction factor by attenuating vesicular stomatitis virus (VSV) replication (PubMed:17670836). {ECO:0000250|UniProtKB:O75807, ECO:0000269|PubMed:11381086, ECO:0000269|PubMed:12606582, ECO:0000269|PubMed:12824288, ECO:0000269|PubMed:16478986, ECO:0000269|PubMed:17670836}.

Molecular Weight: 71.8 kDa

UniProt: P17564

AlphaFold: P17564

Pathways: ER-Nucleus Signaling, Unfolded Protein Response