Hexokinase 1 Protein (HK1) (AA 1-974) (His tag)

Catalog No. ABIN7562918

This Recombinant Hexokinase 1 protein is expressed in HEK-293 Cells.

Quick Overview for Hexokinase 1 Protein (HK1) (AA 1-974) (His tag) (ABIN7562918)

Target: Hexokinase 1 (HK1)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-974

Purification tag / Conjugate: This Hexokinase 1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Hk1 Protein expressed in mammalian cells.

Sequence: MGWGAPLLSR MLHGPGQAGE TSPVPERQSG SENPASEDRR PLEKQCSHHL YTMGQNCQRG QAVDVEPKIR PPLTEEKIDK YLYAMRLSDE ILIDILTRFK KEMKNGLSRD YNPTASVKML PTFVRSIPDG SEKGDFIALD LGGSSFRILR VQVNHEKSQN VSMESEVYDT PENIVHGSGS QLFDHVAECL GDFMEKRKIK DKKLPVGFTF SFPCRQSKID EAVLITWTKR FKASGVEGAD VVKLLNKAIK KRGDYDANIV AVVNDTVGTM MTCGYDDQQC EVGLIIGTGT NACYMEELRH IDLVEGDEGR MCINTEWGAF GDDGSLEDIR TEFDRELDRG SLNPGKQLFE KMVSGMYMGE LVRLILVKMA KESLLFEGRI TPELLTRGKF TTSDVAAIET DKEGVQNAKE ILTRLGVEPS HDDCVSVQHV CTIVSFRSAN LVAATLGAIL NRLRDNKGTP RLRTTVGVDG SLYKMHPQYS RRFHKTLRRL VPDSDVRFLL SESGSGKGAA MVTAVAYRLA EQHRQIEETL SHFRLSKQAL MEVKKKLRSE MEMGLRKETN SRATVKMLPS YVRSIPDGTE HGDFLALDLG GTNFRVLLVK IRSGKKRTVE MHNKIYSIPL EIMQGTGDEL FDHIVSCISD FLDYMGIKGP RMPLGFTFSF PCKQTSLDCG ILITWTKGFK ATDCVGHDVA TLLRDAVKRR EEFDLDVVAV VNDTVGTMMT CAYEEPSCEI GLIVGTGSNA CYMEEMKNVE MVEGNQGQMC INMEWGAFGD NGCLDDIRTD FDKVVDEYSL NSGKQRFEKM ISGMYLGEIV RNILIDFTKK GFLFRGQISE PLKTRGIFET KFLSQIESDR LALLQVRAIL QQLGLNSTCD DSILVKTVCG VVSKRAAQLC GAGMAAVVEK IRENRGLDHL NVTVGVDGTL YKLHPHFSRI MHQTVKELSP KCTVSFLLSE DGSGKGAALI TAVGVRLRGD PTNA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Hexokinase 1 (HK1)

Alternative Name: Hk1

Background: Hexokinase-1 (EC 2.7.1.1) (Hexokinase type I) (HK I) (Hexokinase, tumor isozyme),FUNCTION: Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine (By similarity). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331). {ECO:0000250|UniProtKB:P05708, ECO:0000250|UniProtKB:P19367, ECO:0000269|PubMed:27374331}.

Molecular Weight: 108.3 kDa

UniProt: P17710

AlphaFold: P17710

Pathways: Carbohydrate Homeostasis, Warburg Effect