Cyclin B1 Protein (CCNB1) (AA 1-430) (His tag)
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- Target See all Cyclin B1 (CCNB1) Proteins
- Cyclin B1 (CCNB1)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-430
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Cyclin B1 protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant Ccnb1 Protein expressed in mammalian cells.
- Sequence
- MALRVTRNTK INAENKAKVS MAGAKRVPVT VTAASKPGLR PRTALGDIGN KVSEELQARV PLKREAKTLG TGKGTVKALP KPVEKVPVCE PEVELAEPEP EPELEHVREE KLSPEPILVD NPSPSPMETS GCAPAEEYLC QAFSDVILAV SDVDADDGAD PNLCSEYVKD IYAYLRQLEE EQSVRPKYLQ GREVTGNMRA ILIDWLIQVQ MKFRLLQETM YMTVSIIDRF MQNSCVPKKM LQLVGVTAMF IASKYEEMYP PEIGDFAFVT NNTYTKHQIR QMEMKILRVL NFSLGRPLPL HFLRRASKVG EVDVEQHTLA KYLMELSMLD YDMVHFAPSQ IAAGAFCLAL KILDNGEWTP TLQHYLSYSE DSLLPVMQHL AKNVVMVNCG LTKHMTVKNK YAASKHAKIS TLAQLNCTLV QNLSKAVTKA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Cyclin B1 (CCNB1) (AA 1-433) protein (His tag)
custom-made CCNB1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cyclin B1 (CCNB1) (AA 1-433) protein (GST tag)CCNB1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Cyclin B1 (CCNB1) (AA 1-433) protein (His tag)CCNB1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- Cyclin B1 (CCNB1)
- Alternative Name
- Ccnb1 (CCNB1 Products)
- Background
- G2/mitotic-specific cyclin-B1,FUNCTION: Essential for the control of the cell cycle at the G2/M (mitosis) transition.
- Molecular Weight
- 48.1 kDa
- UniProt
- P24860
- AlphaFold
- P24860
- Pathways
- Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
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