RASGRF1 Protein (AA 1-1262) (His tag)

Catalog No. ABIN7563028

Product Details

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1262

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This RASGRF1 protein is labelled with His tag.

Purpose: Custom-made recombinant Rasgrf1 Protein expressed in mammalian cells.

Sequence: MQKAIRLNDG HVVTLGLLAQ KDGTRKGYLS KRSADNPKWQ TKWFALLQNL LFYFESDSSP RPSGLYLLEG SICKRAPSPK RGTSSKESGE KQQHYFTVNF SNDSQKTLEL RTEDAKDCDE WVAAIARASY KILATEHEAL MQKYLHLLQV VETEKTVAKQ LRQQLEDGEV EIERLKTEVT ITNLIKDNDR IQSSNKAGSA DDEDSDIKKI KKVQSFLRGW LCRRKWKNII QDYIRSPHAD SMRKRNQVVF SMLEAEAEYV QQLHILVNNF LRPLRMAASS KKPPITHDDV SSIFLNSETI MFLHQIFYQG LKARISSWPT LVLADLFDIL LPMLNIYQEF VRNHQYSLQI LAHCKQNRDF DKLLKQYEAK PDCEERTLET FLTYPMFQIP RYILTLHELL AHTPHEHVER NSLDYAKSKL EELSRIMHDE VSETENIRKN LAIERMITEG CEILLDTSQT FVRQGSLMQM SLSEKSKSSR GRLGSLSTKK EGERQCFLFS KHLIICTRGS GGKLHLTKNG VISLIDCTLL DEPENLDDEA KGAGPEIEHL EFKIGVEPKD SLPFTVILVA STRQEKAAWT SDIIQCVDNI RCNGLMMNAF EENSKVTVPQ MIKSDASLYC DDVDIRFSKT MNSCKVLQIR YASVERLLER LTDLRFLSID FLNTFLHSYR VFTNAMVVLD KLINIYRKPM SAIPARSLEL LFSSSHNAKL LYGDAPKSPR ASRKFSSPPP LAIGTSSPSR RRKLSLNIPI ITGGKALELA SLGCSSDSYA NIHSPISPFG KTTLDTGKLC MASSLPKTPE EIDVPATIPE KPGELSASRK HSSDVLKEES EDDQNHSDED NTEVSPVKSP PTPKSFLNRT ITEFPFFNYN NGILMTTCRD LVDNNRSTLS ATSAFAIATA GANEGPSNKE VFRRMSLANT GFSSDQRNID KEFVIRRAAT NRVLNVLRHW VTKHTQDFDT DDTLKYRVIC FLEEVMHDPD LLTQERKAAA NIIRTLTLEE TTEQHSMLEE VILMTEGVKT EPFENHPALE IAEQLTLLDH LVFKSIPYEE FFGQGWMKAE KYERTPYIMK TTKHFNHVSN FIASEIIRNE DISARASAIE KWVAVADICR CLHNYNAVLE ITSSINRSAI FRLKKTWLKV SKQTKSLLDK LQKLVSSDGR FKNLRESLRN CDPPCVPYLG MYLTDLVFIE EGTPNYTEDG LVNFSKMRMI SHIIREIRQF QQTTYKIDPQ PKVIQYLLDE SFMLDEESLY ESSLLIEPKL PT Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Alternative Name: Rasgrf1 (RASGRF1 Products)

Background: Ras-specific guanine nucleotide-releasing factor 1 (Ras-GRF1) (CDC25Mm) (Guanine nucleotide-releasing protein) (GNRP) (Ras-specific nucleotide exchange factor CDC25),FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000269|PubMed:10373510}.

Molecular Weight: 144.1 kDa

UniProt: P27671

AlphaFold: P27671

Pathways: RTK Signaling, Cell Division Cycle, M Phase