TAP2 Protein (AA 1-702) (His tag)

Catalog No. ABIN7563194

This Recombinant TAP2 protein is expressed in HEK-293 Cells.

Quick Overview for TAP2 Protein (AA 1-702) (His tag) (ABIN7563194)

Target: TAP2 (Transporter 2, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP2))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-702

Purification tag / Conjugate: This TAP2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Tap2 Protein expressed in mammalian cells.

Sequence: MALSYLRPWV SLLLADMALL GLLQGSLGNL LPQGLPGLWI EGTLRLGVLW GLLKVGELLG LVGTLLPLLC LATPLFFSLR ALVGGTASTS VVRVASASWG WLLAGYGAVA LSWAVWAVLS PAGVQEKEPG QENRTLMKRL LKLSRPDLPF LIAAFFFLVV AVWGETLIPR YSGRVIDILG GDFDPDAFAS AIFFMCLFSV GSSFSAGCRG GSFLFTMSRI NLRIREQLFS SLLRQDLGFF QETKTGELNS RLSSDTSLMS RWLPFNANIL LRSLVKVVGL YFFMLQVSPR LTFLSLLDLP LTIAAEKVYN PRHQAVLKEI QDAVAKAGQV VREAVGGLQT VRSFGAEEQE VSHYKEALER CRQLWWRRDL EKDVYLVIRR VMALGMQVLI LNCGVQQILA GEVTRGGLLS FLLYQEEVGQ YVRNLVYMYG DMLSNVGAAE KVFSYLDRKP NLPQPGILAP PWLEGRVEFQ DVSFSYPRRP EKPVLQGLTF TLHPGTVTAL VGPNGSGKST VAALLQNLYQ PTGGQLLLDG EPLTEYDHHY LHRQVVLVGQ EPVLFSGSVK DNIAYGLRDC EDAQVMAAAQ AACADDFIGE MTNGINTEIG EKGGQLAVGQ KQRLAIARAL VRNPRVLILD EATSALDAQC EQALQNWRSQ GDRTMLVIAH RLHTVQNADQ VLVLKQGRLV EHDQLRDGQD VYAHLVQQRL EA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TAP2 (Transporter 2, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP2))

Alternative Name: Tap2

Background: Antigen peptide transporter 2 (APT2) (EC 7.4.2.14) (ATP-binding cassette sub-family B member 3) (Histocompatibility antigen modifier 2),FUNCTION: ABC transporter associated with antigen processing. In complex with TAP1 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules. Uses the chemical energy of ATP to export peptides against the concentration gradient. During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle. As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation. {ECO:0000250|UniProtKB:Q03519}.

Molecular Weight: 77.4 kDa

UniProt: P36371

AlphaFold: P36371

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response