RASIP1 Protein (AA 1-961) (His tag)

Catalog No. ABIN7563692

This Recombinant RASIP1 protein is expressed in HEK-293 Cells.

Quick Overview for RASIP1 Protein (AA 1-961) (His tag) (ABIN7563692)

Target: RASIP1 (Ras Interacting Protein 1 (RASIP1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-961

Purification tag / Conjugate: This RASIP1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Rasip1 Protein expressed in mammalian cells.

Sequence: MLSGERKEGG SPRFGKLHLP VGLWINSPRK QLAKLGRRWP SAASVKSSSS DTGSRSSEPL PPPPPPPHVE LRRVGAVKAA GGASGSRAKR ISQLFRGSGA GGAGGPGTPG GAQRWASEKK LPELAAGVAP EPPLPTRAAV PPGVLKIFAS GLASGANYKS VLATERSTAR ELVAEALERY GLTGGRGAGD SGCVDAYALC DALGRPAVGV GGGEWRAEHL RVLADAERPL LVQDLWRARP GWARRFELRG REEARRLEQE AFGAADADGT NAPSWRTQKN RSRAASGGAA LASPGPGSGS GTPTGSGGKE RSENLSLRRS VSELSLQGRR RRQQERRQQA LSMAPGAADA QMVPTDPGDF DQLTQCLIQA PSNRPYFLLL QGYQDAQDFV VYVMTREQHV FGRGGPSSSR GGSPAPYVDT FLNAPDILPR HCTVRAGPEP PAMVRPSRGA PVTHNGCLLL REAELHPGDL LGLGEHFLFM YKDPRSGGSG PARPSWLPAR PGAAPPGPGW AFSCRLCGRG LQERGEALAA YLDGREPVLR FRPREEEALL GEIVRAAASG AGDLPPLGPA TLLALCVQHS ARELELGHLP RLLGRLARLI KEAVWEKIKE IGDRQPENHP EGVPEVPLTP EAVSVELRPL ILWMANTTEL LSFVQEKVLE MEKEADQEGL SSDPQLCNDL ELCDEALALL DEVIMCTFQQ SVYYLTKTLY STLPALLDSN PFTAGAELPG PGAELEAMPP GLRPTLGVFQ AALELTSQCE LHPDLVSQTF GYLFFFSNAS LLNSLMERGQ GRPFYQWSRA VQIRTNLDLV LDWLQGAGLG DIATEFFRKL SIAVNLLCVP RTSLLKASWS SLRTDYPTLT PAQLHHLLSH YQLGPGRGPP PAWDPPPAER DAVDTGDIFE SFSSHPPLIL PLGSSRLRLT GPVTDDALHR ELRRLRRLLW DLEQQELPAN HRHGPPVAST P Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RASIP1 (Ras Interacting Protein 1 (RASIP1))

Alternative Name: Rasip1

Background: Ras-interacting protein 1 (Rain),FUNCTION: Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells. May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation. Negative regulator of amino acid starvation-induced autophagy (By similarity). {ECO:0000250, ECO:0000269|PubMed:19272373, ECO:0000269|PubMed:21396893}.

Molecular Weight: 103.6 kDa

UniProt: Q3U0S6

AlphaFold: Q3U0S6