COBL Protein (AA 1-1337) (His tag)

Catalog No. ABIN7563824

This Recombinant COBL protein is expressed in HEK-293 Cells.

Quick Overview for COBL Protein (AA 1-1337) (His tag) (ABIN7563824)

Target: COBL (Cordon-Bleu WH2 Repeat Protein (COBL))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1337

Purification tag / Conjugate: This COBL protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Cobl Protein expressed in mammalian cells.

Sequence: MDAPRALAAK PPTGRKMKAR APPPPGKPAA QNVHSEQKLP HDATLGSQQS LVYMKEALQN STLDITVVLP SGLEKQSVVS GSHAMMDLLV ELCLQNHLNP SHHVLEIWSS ETQQPLSFKP NTLIGSLNVH TVLLKEKVPE ERVKPGLTKA PEKSVRLVVN YLRTQKAVVR VSPEVPLQNI LPVICAKCEV NPEHVILLRD NVAGEELELS KSLNELGIKE LYAWDNRREM FRKSSLGNDE TDKEKKKFLG FFKANKRSNS KAEHLGLSGA DSDEDPAKSA SGGDLNGCVT TPNSPSLHSR SLTLGPSLSL GNISGVSMKS DMKKRRAPPP PSPKLLGQDK VSEKASLSSQ ADLQKKKRRA PAPPPPQQPP PSPVVPNRKE DKEENRKSTV GVGRQVPQKP PRGTARGPPQ LVLPPPPPYP PPDTDVTEPV TFPGEGAGSE TSELRPKLSL PLGPGSHCSM GGVSQVPAES EETASEDTTE DSGVMSSPSD AISLDSQQDS MRSKDKWSTD QEDGSDQDLA GTPELGPQKS PSWGKSGSGS SILRTEKATM PTNDDEDLFI TGHLHQTLAE LDEDLEGMEE NYETDTSSLT NSVNGVSNHS LQEAIIPDSG VDDIPVTFIG EVSDEPFDSG LFSSRCNNAT TFNTGSIASQ RSHLSPSQTE HSQPFVRTSR KEPDPSPPSQ DNRKRNQPTL ANTSENENPV ETDPTVTSLV SKLLIDDPKA KDKGKVHGSS HSEKTQAGHG INSLRVNPRD GKDESSNSAP PPWSHHGQAL GGSYGLKYGL TTYKIVPPKS EMRCYDRDVS LSTGAIKIDE LGNLVSPHMN GSRTISPPSA VVETDTPPIG KVKEFWRRNS MEKYLNGPAE CTIKRAPSTT ITATPEKPQQ DNGMKAAFTV TTPQQQPASQ EYGAHLEEER SRPQSAVSCS VKVPASNPTD ITFLKPQRRT SSQYVASAIA KKMGPPKVHA DVVRPHKATT EQCHEEAKLA RSPPTRKDDA APNLHSEARQ HEHGTNQSSV CLPSNPGVQL PAGGHPKVEV NSTYGKSSTQ DYPAAVHRNS YFLPGRSSHR DRVSVGQSCG FNEKQTTSNQ KANSTSNFSQ ALDKAHPPPL LLAEARDSGR ILMNGSARTP GNCEPPHSPK ESTLTSYIIL QTEEKPSSLS TDGQDADDTL PSSIFGPKKK FKPVIQRPLP KDVSLHSALM EAIHSSGGRE KLRKTAEQTS EGRPKKPSYV EAESERSALL AAIRGHSGTL SLRKVSSLAS EELQSFRNAA LGAPGLDKPQ QEDLGLPPPP ALPPPPAPAP QAPSASVTVS RFSTGTPSNS VNARQALMDA IRSGTGAARL RKVPLLV Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: COBL (Cordon-Bleu WH2 Repeat Protein (COBL))

Alternative Name: Cobl

Background: Protein cordon-bleu,FUNCTION: Plays an important role in the reorganization of the actin cytoskeleton. Binds to and sequesters actin monomers (G actin). Nucleates actin polymerization by assembling three actin monomers in cross-filament orientation and thereby promotes growth of actin filaments at the barbed end. Can also mediate actin depolymerization at barbed ends and severing of actin filaments. Promotes formation of cell ruffles. Regulates neuron morphogenesis and increases branching of axons and dendrites. Regulates dendrite branching in Purkinje cells. {ECO:0000269|PubMed:17956734, ECO:0000269|PubMed:23223303}.

Molecular Weight: 143.9 kDa

UniProt: Q5NBX1

AlphaFold: Q5NBX1