CRTC1 Protein (AA 1-630) (His tag)

Catalog No. ABIN7564004

Product Details

Target: CRTC1 (CREB Regulated Transcription Coactivator 1 (CRTC1))

Protein Type: Recombinant

Protein Characteristics: AA 1-630

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This CRTC1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Crtc1 Protein expressed in mammalian cells.

Sequence: MATSNNPRKF SEKIALHNQK QAEETAAFEE VMKDLSLTRA ARLQLQKSQY LQLGPSRGQY YGGSLPNVNQ IGSSSVDLAF QTPFQSSGLD TSRTTRHHGL VDRVYRERGR LGSPHRRPLS VDKHGRQADS CPYGTVYLSP PADTSWRRTN SDSALHQSTM TPSQAESFTG GSQDAHQKRV LLLTVPGMED TGAETDKTLS KQSWDSKKAG SRPKSCEVPG INIFPSADQE NTTALIPATH NTGGSLPDLT NIHFPSPLPT PLDPEEPPFP ALTSSSSTGS LAHLGVGGAG QGMNTPSSSP QHRPAVVSPL SLSTEARRQQ AQQVSPTLSP LSPITQAVAM DALSLEQQLP YAFFTQTGSQ QPPPQPQPPP PPPPVSQQQP PPPQVSVGLP QGGPLLPSAS LTRGPQLPPL SVTVPSTLPQ SPTENPGQSP MGIDATSAPA LQYRTSAGSP ATQSPTSPVS NQGFSPGSSP QHTSTLGSVF GDAYYEQQMT ARQANALSRQ LEQFNMMENA ISSSSLYNPG STLNYSQAAM MGLSGSHGGL QDPQQLGYTG HGGIPNIILT VTGESPPSLS KELSSTLAGV SDVSFDSDHQ FPLDELKIDP LTLDGLHMLN DPDMVLADPA TEDTFRMDRL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: CRTC1 (CREB Regulated Transcription Coactivator 1 (CRTC1))

Alternative Name: Crtc1 (CRTC1 Products)

Background: CREB-regulated transcription coactivator 1 (Mucoepidermoid carcinoma translocated protein 1 homolog) (Transducer of regulated cAMP response element-binding protein 1) (TORC-1) (Transducer of CREB protein 1),FUNCTION: Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites (PubMed:29211348). Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated (PubMed:29211348). Acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells (By similarity). In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons. In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock. {ECO:0000250|UniProtKB:Q157S1, ECO:0000250|UniProtKB:Q6UUV9, ECO:0000269|PubMed:17360587, ECO:0000269|PubMed:23699513, ECO:0000269|PubMed:23993098, ECO:0000269|PubMed:29211348}.

Molecular Weight: 66.9 kDa

UniProt: Q68ED7

AlphaFold: Q68ED7