BEND3 Protein (AA 1-825) (His tag)

Catalog No. ABIN7564105

This Recombinant BEND3 protein is produced in HEK-293 Cells.

Quick Overview for BEND3 Protein (AA 1-825) (His tag) (ABIN7564105)

Target: BEND3 (BEN Domain Containing 3 (BEND3))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-825

Purification tag / Conjugate: This BEND3 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Bend3 Protein expressed in mammalian cells.

Sequence: MNSTEISEDV EEVLKNNPVK AEGSDATLDC SRNSRASEKH LLESVLTALH DSSKRKQLDS DGQPDSVPSV KRRRLIPEAL LAGMRTRENS SPCQGNGEPA SRGRSGSCAW PAEEEPSTEA TVPSYKKPLY GISHKIMEKK NPPSGDLLSP YELFEKANSS SGPSPLRLLS ESQKRECGVG VATDGDLNIY FLIQKMFYML NGLTTNMSQL HSKMDLLSLE VSRVKKQVSP SELVAKFQPP PEYQLTASEL KQIAEQSLSC GDLACRLLLQ LFPELFSDVD FSRGCSACGF AAKRKLESLH LQLIRNYVEV YYPNVKDTAV WQAECLPQLN DFFSRFWAQR EMEDSQPGGQ VTNFFEADQV DAGHFLDNKD QEEALSLDRS STIASDHVVD TQDLTEFLDE ASSPGEFAVF LLHRLFPELF DHRKLGEQYS CYGDGGKQEL DPQRLQIIRN YTEIYFPDMQ EEEAWLQQCA QRINDELEGL GLEGGSEGEA PRDDCYDSSS LPDDISVVKV EDNFEGERPG RRSKKIWLVP IDFDKLEIPQ PDFEMPGSDC LLSKEQLRSI YESSLSIGNF ASRLLVHLFP ELFTHENLRK QYNCSGSLGK KQLDPARIRL IRHYVQLLYP RAKNDRVWTL EFVGKLDERC RRRDTEQRRS YQQQRKVHVP GPECRDLASY AINPERFREE FEGPPLPPER SSKDFCKIPL DELVVPSPDF PVPSPYLLSD KEVREIVQQS LSVGNFAARL LVRLFPELFT TENLRLQYNH SGACNKKQLD PTRLRLIRHY VEAVYPVEKM EEVWHYECIP SIDERCRRPN RKKCDILKKA KKVEK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: BEND3 (BEN Domain Containing 3 (BEND3))

Alternative Name: Bend3

Background: BEN domain-containing protein 3,FUNCTION: Transcriptional repressor which associates with the NoRC (nucleolar remodeling complex) complex and plays a key role in repressing rDNA transcription. The sumoylated form modulates the stability of the NoRC complex component BAZ2A/TIP5 by controlling its USP21-mediated deubiquitination (By similarity). Binds to unmethylated major satellite DNA and is involved in the recruitment of the Polycomb repressive complex 2 (PRC2) to major satellites (PubMed:25457167). Stimulates the ERCC6L translocase and ATPase activities (By similarity). {ECO:0000250|UniProtKB:Q5T5X7, ECO:0000269|PubMed:25457167}.

Molecular Weight: 94.2 kDa

UniProt: Q6PAL0

AlphaFold: Q6PAL0