HERC4 Protein (AA 1-1057) (His tag)

Catalog No. ABIN7564109

This Recombinant HERC4 protein is expressed in HEK-293 Cells.

Quick Overview for HERC4 Protein (AA 1-1057) (His tag) (ABIN7564109)

Target: HERC4 (Hect Domain and RLD 4 (HERC4))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1057

Purification tag / Conjugate: This HERC4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Herc4 Protein expressed in mammalian cells.

Sequence: MLCWGNASYG QLGLGGIDEE IVLEPRRSDF FVNKKVRDVG CGLRHTVFVL DDGTVYTCGC NDLGQLGHEK SRKKPEQVVA LDAQNIVAVA CGEAHTLALN DKGQVYAWGL DSDGQLGLQG SEECIRVPRN IKSLSDIQIV QVACGYYHSL ALSKASEVFC WGQNKYGQLG LGIDCQKQTS PQLIKSLLGI PFMQVAAGGA HSFVLTLSGA IFGWGRNKFG QLGLNDENDR YVPNLLKSLR SQKIVYICCG EDHTAALTKE GGVFTFGAGG YGQLGHNSTS HEINPRKVFE LMGSIVTQVA CGRQHTSAFV PSSGRIYSFG LGGNGQLGTG STSNRKSPFT VKGNWFSYNG QCPQDIGSED YFCVKRIFSG GDQSFSHYSS PQNCGPPDDF RCSDPSKQIW TVNEALIQKW LSYPSGRFPV EIANEIDGTF SSSGCLNGSF LAISNDDHYR TGTRFSGVDM NAARLLFHKL IQPDHPQISQ QVAASLEKNL IPKLTSSLPD VEALRFYLTL PECPLMSDCN NFTTIAIPFG TALVNLEKAP LKVLENWWSV LEPPLFLKIV ELFKEVVVHL LKLYKIGIPP SERRIFNSFL HTALKVLEIL HRVNEKTGQL IQYDKFYIHE VQELIDIRND YINWVQQQAY GVDVSHGVTE LADIPVTICT YPFVFDAQAK TTLLQTDAVL QMQMAIDQAH RQNVSSLFLP VIESVNPCLI LVVRRENIVG DAMEVLRKTK NIDYKKPLKV IFVGEDAVDA GGVRKEFFLL IMRELLDPKY GMFRYYEDSR LIWFSDKTFE DSDLFHLIGV ICGLAIYNFT IVDLHFPLAL YKKLLKRKPS LDDLKELMPA VGRSMQQLLD YPEDDIEETF CLNFTITVEN FGATEVKELV LNGADTAVNR QNRQEFVDAY VDYIFNKSVA SLFDAFHAGF HKVCGGKVLL LFQPNELQAM VIGNTNYDWK ELEKNTEYKG EYWADHPTIK IFWEVFHELP LEKKKQFLLF LTGSDRIPIL GMKSLKLVIQ STGGGESYLP VSHTCFNLLD LPKYTEKETL RCKLIQAIDH NEGFSLI Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: HERC4 (Hect Domain and RLD 4 (HERC4))

Alternative Name: Herc4

Background: Probable E3 ubiquitin-protein ligase HERC4 (EC 2.3.2.26) (HECT domain and RCC1-like domain-containing protein 4) (HECT-type E3 ubiquitin transferase HERC4),FUNCTION: Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates. {ECO:0000269|PubMed:17967448}.

Molecular Weight: 118.4 kDa

UniProt: Q6PAV2

AlphaFold: Q6PAV2