IARS Protein (AA 1-1262) (His tag)

Catalog No. ABIN7564428

Recombinant IARS protein expressed in HEK-293 Cells.

Quick Overview for IARS Protein (AA 1-1262) (His tag) (ABIN7564428)

Target: IARS (Isoleucyl-tRNA Synthetase (IARS))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1262

Purification tag / Conjugate: This IARS protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Iars1 Protein expressed in mammalian cells.

Sequence: MVQQVPENIS FPAEEEKILE FWSKHNCFQE CLKQSKLRPK FTFYDGPPFA TGLPHYGHIL AGTIKDIVTR YAHQSGFHVD RRFGWDCHGL PVEYEIDKTL GIKGPEDVAK MGIAEYNKQC RAIVMRYSAE WKSTVTRLGR WIDFDNDYKT LYPQFMESVW WVFKQLYDKG LVYRGVKVMP FSTACGTPLS NFESNQNYKD VQDPSVFVTF PLEEDENTSL VAWTTTPWTL PSNLALCVNP EIQYVKIKDV ARGKLFILTE ARLSALYKQE SDYEILERFP GASLKGKKYK PLFDYFIKCK ENGAFTVLVD HYVKDEEGTG VVHQAPYFGA DDHRVCMDFN IIQKDSVPVC PVDASGCFTE EVTHFVGQYV KDADKNIIRM LKEQGRLLAA GTFTHSYPFC WRSDTPLIYK SVPSWFVRVE PMVDQLLKNN DLCYWVPEFV REKRFGNWLK EARDWAISRN RYWGTPIPLW VSEDLEEVVC IGSVAELEEL SGTKISDLHR ESIDHLTIPS RCGKAPLRRV SEVFDCWFES GSMPYAQVHY PFESKREFED AFPADFIAEG IDQTRGWFYT LLVLATALFG QPPFKNVIVN GLILASDGQK MSKRKKNYPD PVSIIDKYGA DALRLYLINS PVVRAENLRF KEEGVRDVLK DVLLPWYNAY RFFIQNVFRL HKEEEVKFLY NEHTVRESPN ITDRWVLSFM QSLLGFFETE MAAYRLYTVV PRLVKFVDIL TNWYVRMNRR RLKGESGVED CVMALETLFS VLLSLCRLMA PYTPFLTELM YQNLKLLIDP ASLRDKDTLS IHYLMLPRVR EELIDKKTEN AVSRMQSVIE LGRVIRDRKT IPIKYPLKEI VVIHQDPEAL EDIRSLEKYI IEELNVRKVT LSTDKNKYGI RLRAEPDHMV LGKRLKGAFK AVMMAIKRLS NEELERFQKS GSIVVEGHEL HEEDIRLMYT FDQATGGTAQ FEAHSDAQAL VLLDVTPDQS MVDEGMAREV INRIQKLRKK CNLVPTDEIT VYYNAKSEGR YLNNVIESHT DFIFATIKAP LKPYPVPTSD NILIQEQTQL KGSELEITLT KGSCVPGPAC AYVNLNICAN GTEQGGVLLL ENPKGDNQLN LVKLKTVVTS VFGVKNAKLS VFHGETEIQN QTDLLSLSGR TLCVTAGASP SPISSPSTLL CQYLNLQLLN AEPQECLTGT VGTLLLENPL GQNGLTHQGL VHEAAKVFGL RSRRLRLFLN ETQTQEITED IPMKTLNMKT VYVSVLPTTA DG Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: IARS (Isoleucyl-tRNA Synthetase (IARS))

Alternative Name: Iars1

Background: Isoleucine--tRNA ligase, cytoplasmic (EC 6.1.1.5) (Isoleucyl-tRNA synthetase) (IRS) (IleRS),FUNCTION: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. {ECO:0000269|PubMed:12060739}.

Molecular Weight: 144.3 kDa

UniProt: Q8BU30

AlphaFold: Q8BU30