Strumpellin Protein (AA 1-1159) (His tag)

Catalog No. ABIN7564506

Recombinant Strumpellin protein expressed in HEK-293 Cells.

Quick Overview for Strumpellin Protein (AA 1-1159) (His tag) (ABIN7564506)

Target: Strumpellin (WASHC5) (WASH Complex Subunit 5 (WASHC5))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1159

Purification tag / Conjugate: This Strumpellin protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Washc5 Protein expressed in mammalian cells.

Sequence: MLDFLAENNL CGQAILRIVS CGNAIIAEVL RLSEFIPAVF LLKDRADQQR YGDIIFDFSY FKGPEFWESK LEAKPELQDL DEEFRENNIE IVTRFYLAFQ SVHKYIVDLN RYLDDLNEGV YIQQTLETVL LSEDGKQLLC EALYLYGVML LVIDQKIEGE VRERMLVSYY RYSAARSSAD SNMDDICKLL RSTGYSSQPG AKRPPNYPES YFQRVPINET FISMVIGRLR SDDIYNQVSA YPLPEHRSTA LANQAAMLYV ILYFEPSILH THQAKMREIV DKYFPDNWVI SIYMGITVNL ADAWEPYKAA KTALNNTLDL ANVKEQASRY ASVSDRVRAQ VQQFLKEGYL REEVLLDNIP RLLNCLRDCN VAIRWLMLHT ADSACDPNNK RLRQIKDQIL ADSRYNPKIL FQLLLDTAQF EFILKEMFKQ MLSEKQSKWE HYKKEGSERM TELADVFSGV KPLTRVEKNE NLQAWFREIS KQILSLNYDD STAAGRKTVQ LIQALEEVQE FHQLESNLQV CQFLADTRKF LHQMIRTINI KEEVLITVQI IGDLSFAWQL IDSFTSIMQE SIRVNPSMVT KLRATFLKLA SALDLPLLRI NQANSPDLLS VSQYYSGELV SYVRKVLQII PESMFTSLLK IIKLQTHDIM EVPTRLDKDK LRDYAQLGPR YEVAKLTHAI SIFTEGILMM KTTLVGIIKV DPKQLLEDGI RKELVKRVAF ALHRGLIFNP RAKPSELMPK LKELGATMDG FHRSFEYIQD YVSIYGLKIW QEEVSRIINY NVEQECNNFL RTKIQDWQSM YQSTHIPIPK FAPVDESITF IGRLCREILR ITDPKMTCYI DQLNTWYDVK THQEVTSSRL FSEIQTTLGT FGLNGLDRLL CFMIVKELQN FLSMFQKIIL KERTVQETLK MLMSAVNPLK SIVANSSKVY LSAITKTQKI WSAYLEAIMK VGQMQILRQQ IANELNSSCR FDSRHLAAAL DNLNKALLAD IEAHYRDPSL PYPKEDNTLL YEITAYLEAA GIHNPLNKIY ITTKRLPYFP IVNFLFLIAQ LPKLQYNKNL GMVCRKPADP VDWPPLVLGL LTLLKQFHSR YTEQFLALIG QFIRSTMEQC TSQKMPEMPA DAVGALLFLE DYVRYTKLPR RVAEAHVPNF IFDEFRTVL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Strumpellin (WASHC5) (WASH Complex Subunit 5 (WASHC5))

Alternative Name: Washc5

Background: WASH complex subunit 5 (WASH complex subunit strumpellin),FUNCTION: Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. May be involved in axonal outgrowth. Involved in cellular localization of ADRB2. Involved in cellular trafficking of BLOC-1 complex cargos such as ATP7A and VAMP7 (By similarity). Involved in cytokinesis and following polar body extrusion during oocyte meiotic maturation (PubMed:24998208). {ECO:0000250|UniProtKB:Q12768, ECO:0000269|PubMed:24998208}.

Molecular Weight: 134.1 kDa

UniProt: Q8C2E7

AlphaFold: Q8C2E7