NGEF Protein (AA 1-710) (His tag)

Catalog No. ABIN7564588

This Recombinant NGEF protein is produced in HEK-293 Cells.

Quick Overview for NGEF Protein (AA 1-710) (His tag) (ABIN7564588)

Target: NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-710

Purification tag / Conjugate: This NGEF protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ngef Protein expressed in mammalian cells.

Sequence: METKNSEDWG KPQRKSESSS RKSNHGPAEM RPALPPENRE APETGEETQN EEPRRLIPIQ RHSLFNRAVR HRHKARSTSE RRASDQADLP KMGKSVNERS AFNLPQGRLS PWRTPAQRDT GAQEASESSS TPGNGTTPEE CPALTDSPTT LTEALQMIHP IPADSWRNLI EQIGLLYQEY RDKSTLQEIE TRRQQDAEIQ GNSDGSQVGE DAGEEEEEEE EGEEEELASP PERRALPQIC LLSNPHSRFN LWQDLPEIQS SGVLDILQPE EIRLQEAMFE LVTSEASYYK SLNLLVSHFM ENERLKKILH PSEAHILFSN VLDVMAVSER FLLELEHRME ENIVISDVCD IVYRYAADHF SVYITYVSNQ TYQERTYKQL LQEKAAFREL IAQLELDPKC KGLPFSSFLI LPFQRITRLK LLVQNILKRV EERSEREGTA LDAHKELEMV VKACNEGVRK MSRTEQMISI QKKMEFKIKS VPIISHSRWL LKQGELQQMS GPKTSRTLRT KKLFREIYLF LFNDLLVICR QIPGDKYQVF DSAPRGLLRV EELEDQGQTL ANVFILRLLE NADDREATYM LKASSQSEMK RWMTSLAPNR RTKFVSFTSR LLDCPQVQCV HPYVAQQPDE LTLELADILN ILEKTEDGWI FGERLHDQER GWFPSSMTEE ILNPKIRSQN LKECFRVHKM EDPQRSQNKD RRKLGSRNRQ Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))

Alternative Name: Ngef

Background: Ephexin-1 (Eph-interacting exchange protein) (Neuronal guanine nucleotide exchange factor),FUNCTION: Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension. {ECO:0000269|PubMed:11336673, ECO:0000269|PubMed:15848799, ECO:0000269|PubMed:17143272}.

Molecular Weight: 82.2 kDa

UniProt: Q8CHT1

AlphaFold: Q8CHT1

Pathways: Neurotrophin Signaling Pathway