DEPDC1 Protein (AA 1-804) (His tag)

Catalog No. ABIN7564592

This Recombinant DEPDC1 protein is expressed in HEK-293 Cells.

Quick Overview for DEPDC1 Protein (AA 1-804) (His tag) (ABIN7564592)

Target: DEPDC1 (DEP Domain Containing 1 (DEPDC1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-804

Purification tag / Conjugate: This DEPDC1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Depdc1a Protein expressed in mammalian cells.

Sequence: MERQGAPLGP YRATKLWNEV TTSFRVGMPL RKHRQHLKKY SNCFTAVEAI DWLYDLLRSN SNFGPEVTRQ QTIQLLRKFL KNHVIEDIKG RWGSESLDDN NQLFRFPATS PLKTFPQRHT EIKKTNTEYF SKDKDGIFKL RNLSQKTSKK RGLHFSQENT EKINHERITN EDLEIAPDNQ EISQEDVEEV WRYVIMIYLQ TILSLPSIEE LLNPNQVIPQ YIMYNMANTS KHGVVILQDK SDDLPHWVLS AMKCLANWPR SNDTNNLTYV GFERDVFKTI ADYFLNLPEP LLTFEYYELF VNILVVCGYI TVSDRTSGIH KIQDDPRSSK IHDLSNLNSF KSTECLLLSL LYKDKSNEEL DSTKRLQRND QGFQERCAKK MQLDNLRNRR ASANDIMGGS CHNLIGLSNT NALSSNIKPR CSSLEGIVDR PVNSSEKKSS IFYQSVLNIE EQNSKQSLVS APKQTPLFNL HSDENAQQPH CVGFNRTSAL TVQDQEELCN EKYKSKQLCR SQSLLLRSST RQNSCINKPV AEIIMKPNVG QGSTSELGES STTINKRLCK STIELSEKSL PPAASVLTGT QSLLQPHLER VAINALQLCC LLLPPPNRRK LQLLMRMISR MSQNVDMPKL HEQIGTRSLM INTFSRCVLC CAEEVDLDEL LASRLVSFLM DHHQEILQVP TYLQAAVEKH LDYIKKGNVK NHGDGLVVPL PTYSYCKQIS AKEFDEQKIS TSQAAIAELL ENIVRSKSLS LKEKRRKLKQ KEYPLIYQKR FPTTESEAAL FDDKPTIKQP MLNLRNPKLH SLRY Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DEPDC1 (DEP Domain Containing 1 (DEPDC1))

Alternative Name: Depdc1a

Background: DEP domain-containing protein 1A,FUNCTION: May be involved in transcriptional regulation as a transcriptional corepressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells (By similarity). {ECO:0000250}.

Molecular Weight: 92.2 kDa

UniProt: Q8CIG0

AlphaFold: Q8CIG0