Sphingomyelin Synthase 1 Protein (SGMS1) (AA 1-419) (His tag)

Catalog No. ABIN7564736

This Recombinant Sphingomyelin Synthase 1 protein is expressed in HEK-293 Cells.

Quick Overview for Sphingomyelin Synthase 1 Protein (SGMS1) (AA 1-419) (His tag) (ABIN7564736)

Target: Sphingomyelin Synthase 1 (SGMS1)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-419

Purification tag / Conjugate: This Sphingomyelin Synthase 1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Sgms1 Protein expressed in mammalian cells.

Sequence: MLSARTMKEV VYWSPKKVAD WLLENAMPEY CEPLEHFTGQ DLINLTQEDF KKPPLYRVSS DNGQRLLDMI ETLKMEHHME AHKNGHANGH LSIGVDIPNP DGSFSIKTKP NGMPNGFRKE MIKIPMPEPE RSQYPMEWGK TFLAFLYALS CFVLTTVMIS VVHERVPPKE VQPPLPDTFF DHFNRVQWAF SICEINGMIL VGLWLFQWLL LKYKSIISRR FFCIVGTLYL YRCITMYVTT LPVPGMHFNC SPKLFGDWEA QVRRIMKLIA GGGLSITGSH NMCGDYLYSG HTVMLTLTYL FIKEYSPRRL WWYHWICWLL SVVGIFCILL AHDHYTVDVV VAYYITTRLF WWYHTMANQQ VLKEASQMNL LARVWWYRPF QYFEKNVQGI VPRSYHWPFP WPVVHLSRQV KYSRLVNDT Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Sphingomyelin Synthase 1 (SGMS1)

Alternative Name: Sgms1

Background: Phosphatidylcholine:ceramide cholinephosphotransferase 1 (EC 2.7.8.27) (Protein Mob) (Sphingomyelin synthase 1) (Transmembrane protein 23),FUNCTION: Major sphingomyelin synthase at the Golgi apparatus (By similarity). Catalyzes the reversible transfer of phosphocholine moiety in sphingomyelin biosynthesis: in the forward reaction transfers phosphocholine head group of phosphatidylcholine (PC) on to ceramide (CER) to form ceramide phosphocholine (sphingomyelin, SM) and diacylglycerol (DAG) as by-product, and in the reverse reaction transfers phosphocholine from SM to DAG to form PC and CER. The direction of the reaction depends on the levels of CER and DAG in Golgi membranes (By similarity). Does not use free phosphorylcholine or CDP-choline as donor. Regulates receptor-mediated signal transduction via mitogenic DAG and proapoptotic CER, as well as via SM, a structural component of membrane rafts that serve as platforms for signal transduction and protein sorting (PubMed:22580896, PubMed:16879426) (By similarity). Plays a role in secretory transport via regulation of DAG pool at the Golgi apparatus and its downstream effects on PRKD1 (By similarity). {ECO:0000250|UniProtKB:Q86VZ5, ECO:0000269|PubMed:16879426, ECO:0000269|PubMed:22580896}., FUNCTION: (Microbial infection) Contributes to the brain SM production for Japanese encephalitis virus attachment and infection. {ECO:0000269|PubMed:27892528}.

Molecular Weight: 49.3 kDa

UniProt: Q8VCQ6

AlphaFold: Q8VCQ6

Pathways: Cellular Response to Molecule of Bacterial Origin