ELP3/KAT9 Protein (AA 1-547) (His tag)

Catalog No. ABIN7564968

Recombinant ELP3/KAT9 protein expressed in HEK-293 Cells.

Quick Overview for ELP3/KAT9 Protein (AA 1-547) (His tag) (ABIN7564968)

Target: ELP3/KAT9 (ELP3) (Elongator complex protein 3 (ELP3))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-547

Purification tag / Conjugate: This ELP3/KAT9 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Elp3 Protein expressed in mammalian cells.

Sequence: MRQKRKGDLS PAELMMLTIG DVIKQLVEAH EQGKDVDLNK MKTKTAAKYG LASQPRLVDI IAAVPPHYRK ILIPKLKAKP VRTASGIAVV AVMCKPHRCP HISFTGNICI YCPGGPDSDF EYSTQSYTGY EPTSMRAIRA RYDPFLQTRH RIEQLKQLGH SVDKVEFIVM GGTFMALPEE YRDYFIRSLH DALSGHTSNN IHEAIKYSER SFTKCVGITI ETRPDYCMKR HLSDMLTYGC TRLEIGVQSV YEDVARDTNR GHTVKAACES FHLAKDSGFK VVTHMMPDLP NVGLERDIEQ FIEFFENPAF RPDGLKLYPT LVIRGTGLYE LWKSGRYRSY SPSDLIELVA RILALVPPWT RVYRVQRDIP MPLVSSGVEH GNLRELAFAR MKDLGIQCRD VRTREVGIQE IHHRVRPYQV ELVRRDYVAN GGWETFLSYE DPDQDILIGL LRLRKCSEET FRFELGGGVS IVRELHVYGS VVPVSSRDPT KFQHQGFGML LMEEAERIAR EEHGSGKMAV ISGVGTRNYY RKIGYRLQGP YMVKMLK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ELP3/KAT9 (ELP3) (Elongator complex protein 3 (ELP3))

Alternative Name: Elp3

Background: Elongator complex protein 3 (EC 2.3.1.-) (tRNA uridine(34) acetyltransferase),FUNCTION: Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (By similarity). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins, such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase (By similarity). Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (PubMed:19185337). Required for acetylation of GJA1 in the developing cerebral cortex (PubMed:28507509). {ECO:0000250|UniProtKB:D5VRB9, ECO:0000250|UniProtKB:Q9H9T3, ECO:0000269|PubMed:19185337, ECO:0000269|PubMed:22854966, ECO:0000269|PubMed:28507509}.

Molecular Weight: 62.4 kDa

UniProt: Q9CZX0

AlphaFold: Q9CZX0