MAGI3 Protein (AA 1-1476) (His tag)

Catalog No. ABIN7565036

This Recombinant MAGI3 protein is expressed in HEK-293 Cells.

Quick Overview for MAGI3 Protein (AA 1-1476) (His tag) (ABIN7565036)

Target: MAGI3 (Membrane Associated Guanylate Kinase, WW and PDZ Domain Containing 3 (MAGI3))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1476

Purification tag / Conjugate: This MAGI3 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Magi3 Protein expressed in mammalian cells.

Sequence: MSKTLKKKKH WLSKVQECAV SWAGPPGDLG AEIRGGAERG EFPYLGRLRD EAGGGGGTCC VVSGKAPSPG DVLLEVNGTP VSGLTNRDTL AVIRHFREPI RLKTVKPGKV INKDLRHYLS LQFQKGSIDH KLQQVIRDNL YLRTIPCTTR APRDGEVPGV DYNFISVEQF KALEESGALL ESGTYDGNFY GTPKPPAEPS PFQPDPVDQV LFDNEFDTES QRKRTTSVSK MERMDSSLPE EEEDEDKEAV NGSGSMETRE MHSETSDCWM KTVPSYNQTN SSMDFRNYMM RDENLEPLPK NWEMAYTDTG MIYFIDHNTK TTTWLDPRLC KKAKAPEDCE DGELPYGWEK IEDPQYGTYY VDHLNQKTQF ENPVEEAKRK KQLGQAEIHS AKTDVERAHF TRDPSQLKGV LVRASLKKST MGFGFTIIGG DRPDEFLQVK NVLKDGPAAQ DGKIAPGDVI VDINGNCVLG HTHADVVQMF QLVPVNQYVN LTLCRGYPLP DDSEDPVVDI VAATPVINGQ SLTKGETCMN TQDFKLGAMV LDQNGKSGQI LASDRLNGPS ESSEQRASLA SSGSSQPELV TIPLIKGPKG FGFAIADSPT GQKVKMILDS QWCQGLQKGD IIKEIYHQNV QNLTHLQVVE VLKQFPVGAD VPLLILRGGP CSPTKTAKTK TDTKENSGSL ETINEPIPQP MPFPPSIIRS GSPKLDPSEV YLKSKTLYED KPPNTKDLDV FLRKQESGFG FRVLGGDGPD QSIYIGAIIP LGAAEKDGRL RAADELMCID GIPVKGKSHK QVLDLMTTAA RNGHVLLTVR RKIFYGEKQP EDESHQAFSQ NGSPRLNRAE LPTRSAPQEA YDVTLQRKEN EGFGFVILTS KSKPPPGVIP HKIGRVIDGS PADRCGGLKV GDHISAVNGQ SIVDLSHDNI VQLIKDAGVT VTLTVVAEEE HHGPPSGTNS ARQSPALQHR PMGQAQANHI PGDRIALEGE IGRDVCSSYR HSWSDHKHLA QPDTAVISVV GSRHNQSLGC YPVELERGPR GFGFSLRGGK EYNMGLFILR LAEDGPAIKD GRIHVGDQIV EINGEPTQGI THTRAIELIQ AGGNKVLLLL RPGTGLIPDH GDWDTNSPSS SNVIYDEQPP PLPSSHSAST FEESHVPATE DSLTRVQICE KAEELKDTVQ EKKSTLNGSQ PEMKYQSVHK TMSKKDPPRG SGHGEKSRLK GENGVTRRGR SASPKKSVNR HSEEHLEKIP RPLKSDPKEK SRDRSLSPRK GESKGRLTIK AGSGQDPYRK DRGRSSSPKK QQKIGGNSLS NTEGKLSEAG SRRAAGHPRD STEQLPDGRE KSGVSRKDLK QSQPGKTRTK SPEKKSSKVD ETSLPSKKTS STAGRVVSEK EKGKKPTAGE TSRETVEHTQ ISAKQLKQEA QEKTALGNAD DHKGRESEVT DRCRERAGCT PQSSSLVKKA PITPGPWRVP RANKVTGTTG MADKQL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MAGI3 (Membrane Associated Guanylate Kinase, WW and PDZ Domain Containing 3 (MAGI3))

Alternative Name: Magi3

Background: Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 (Membrane-associated guanylate kinase inverted 3) (MAGI-3),FUNCTION: Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2. {ECO:0000269|PubMed:15195140, ECO:0000269|PubMed:15652357}.

Molecular Weight: 161.7 kDa

UniProt: Q9EQJ9

AlphaFold: Q9EQJ9