NFKBIZ Protein (AA 1-728) (His tag)

Catalog No. ABIN7565067

Recombinant NFKBIZ protein expressed in HEK-293 Cells.

Quick Overview for NFKBIZ Protein (AA 1-728) (His tag) (ABIN7565067)

Target: NFKBIZ (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, zeta (NFKBIZ))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-728

Purification tag / Conjugate: This NFKBIZ protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Nfkbiz Protein expressed in mammalian cells.

Sequence: MIVDKLLDDS RGGEGLLDAA GDCGLMTSPL NLAYFYGASP PSAPGAGDTG YLSAVPSAPG SPGSDSSDFS STSSVSSCGA VESRPRGGAR AERPQVEPHM GVGRQQRGPF QGVRVKNSVK ELLLHIRSNK QKASGQPVDE FKTQSVNIEQ LTDLKSAVSA VGKRKGPDPL SDGPVCKRPA LLPSHFVTSP QTPTPGESME DVRHSESKLD SSAALLQNII NIKNECNPVS LNTVQVSWMS PTVPQNSPRD QCQDFHGGQA FSPPQKYQPF QVSGSPQMMD QASMYQYSPQ TQNMQQPPPL PPQQQHQQNY PHNSPLQFSP YSRMSQSPKY DSNLFDTHEP QFCTGQSFVS LLTGPGEPES LAVPVPAPTS IPPQTETQLQ TFSLMPSNAC EAVVGVHDVG SHSLGTSLSL QNIMGSPMNT TQLGKSFFQW QVEQEESKLA NIPQDQFLAR DGDGDTFLHI AVAQGRRALS YVLARKMNAL HMLDIKEHNG QSAFQVAVAA NQHLIVQDLV NLGAQVNTTD CWGRTPLHVC AEKGHSQVLQ AIQKGAVRSN QFVDLEATNY DGLTPLHCAV VAHNAVVHEL QRNRQSHSPE VQDLLLRNKS LVDTIKCLIQ MGAAVEAKDR KSGRTALHLA AEEANLELIR LFLELPSCLS FVNAKAYNGN TALHVAASLQ YRVTQLDAVR LLMRKGADPS TRNLENEQPV HLVPDGPVGE QIRRILKGKS IQQRAPPY Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NFKBIZ (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, zeta (NFKBIZ))

Alternative Name: Nfkbiz

Background: NF-kappa-B inhibitor zeta (I-kappa-B-zeta) (IkB-zeta) (IkappaBzeta) (IL-1 inducible nuclear ankyrin-repeat protein) (INAP) (Molecule possessing ankyrin repeats induced by lipopolysaccharide) (MAIL),FUNCTION: Involved in regulation of NF-kappa-B transcription factor complexes (PubMed:11356851, PubMed:15241416, PubMed:15618216, PubMed:17447895). Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation (PubMed:11356851, PubMed:15241416, PubMed:15618216, PubMed:17447895). Inhibits DNA-binding of RELA and NFKB1/p50, and of the NF-kappa-B p65-p50 heterodimer and the NF-kappa-B p50-p50 homodimer (PubMed:11356851, PubMed:15241416, PubMed:15618216, PubMed:17447895). Seems also to activate NF-kappa-B-mediated transcription (PubMed:11356851, PubMed:15241416, PubMed:15618216, PubMed:17447895). In vitro, upon association with NFKB1/p50 has transcriptional activation activity and, together with NFKB1/p50 and RELA, is recruited to LCN2 promoters (By similarity). Promotes transcription of LCN2 and DEFB4 (By similarity). Is recruited to IL-6 promoters and activates IL-6 but decreases TNF-alpha production in response to LPS (PubMed:11086164, PubMed:25107474). Seems to be involved in the induction of inflammatory genes activated through TLR/IL-1 receptor signaling (PubMed:11086164, PubMed:25107474). Involved in the induction of T helper 17 cells (Th17) differentiation upon recognition of antigen by T cell antigen receptor (TCR) (PubMed:25282160). {ECO:0000250|UniProtKB:Q9BYH8, ECO:0000269|PubMed:11086164, ECO:0000269|PubMed:11356851, ECO:0000269|PubMed:15241416, ECO:0000269|PubMed:15618216, ECO:0000269|PubMed:17447895, ECO:0000269|PubMed:25107474, ECO:0000269|PubMed:25282160}.

Molecular Weight: 79.0 kDa

UniProt: Q9EST8

AlphaFold: Q9EST8

Pathways: NF-kappaB Signaling