Espin Protein (ESPN) (AA 1-871) (His tag)

Catalog No. ABIN7565069

Product Details

Target: Espin (ESPN)

Protein Type: Recombinant

Protein Characteristics: AA 1-871

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This Espin protein is labelled with His tag.

Purpose: Made-to-order recombinant Espn Protein expressed in mammalian cells.

Sequence: MALEQALQAA RRGDLDVLRS LHAAGLLGPS LRDSLDALPV HHAARSGKLH CLRYLVEEVA LPAVSRARNG ATPAHDAAAT GYLSCLQWLL TQGGCRVQEK DNSGATVLHL AARFGHPDVV KWLLYQGGAN SAITTDTGAL PIHYAAAKGD LPSLKLLVGH YPEGVNAQTN NGATPLYLAC QEGHLEVTKY LVQECSADPH LRAQDGMTPL HAAAQMGHNP VLVWLVSFAD VSFSEQDHDG ATAMHFAASR GHTKVLSWLL LHGAEISQDL WGGTPLHDAA ENGELECCQI LAVNGAGLDV RDHDGYTAAD LAEFNGHTHC SRYLRTVQTL SLEHRVLSRD QSMDLEAKQL DSGMSSPNTT MSVQPMTFDL GSPTSTFSNY DSCSSSHSSS KGQRSNRGIP GARAADLQSY MDMLNPEKSL PRGKLGKPSP PPPPPPPPPS FPPPPPPTGT QPPPPPPGYP APNPPVGLHL NNIYMQTKNK LRHVEVDSLK EPKVELNDQF AQPSSGDGHS GLHRQDSGLL RQDSELLHRQ ELLRHSTGLR RQDSDRKQRS FSKQPSTGDY YRQLGRSPGE PLAARPGMAH SEEAALLPGN HVHNGCSADS KASRELPPPP PPPPLPEALS SPPPAPPLPI EGAGAACGQR RSSSSTGKVR VLRHRKSTKS FNMMSPTGDN SELLAEIKAG KSLKPTPQSK GLTTVFSGSG QPASQPESPQ PLVSPAPSRT RSPTPPASGS QPLLNGSVVP APPATPAPGV HLDVEALIPT LDEQGRPIPE WKRQVMVRKL QQKMQEEEEQ RRKEEEEEAR LASLPAWRRD ILRKKLEEER EQKRKEEERQ KLEEIQRAKE QSEKLRTLGY DEAKLAPWQR QVILKKGEIP K Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Espin (ESPN)

Alternative Name: Espn (ESPN Products)

Background: Espin (Ectoplasmic specialization protein),FUNCTION: Multifunctional actin-bundling protein. Plays a major role in regulating the organization, dimension, dynamics and signaling capacities of the actin filament-rich microvilli in the mechanosensory and chemosensory cells (PubMed:14657236, PubMed:15190118). Required for the assembly and stabilization of the stereociliary parallel actin bundles. Plays a crucial role in the formation and maintenance of inner ear hair cell stereocilia (PubMed:21455486). Involved in the elongation of actin in stereocilia (PubMed:19287378, PubMed:22264607). In extrastriolar hair cells, required for targeting MYO3B to stereocilia tips, and for regulation of stereocilia diameter and staircase formation (PubMed:26926603). {ECO:0000269|PubMed:14657236, ECO:0000269|PubMed:15190118, ECO:0000269|PubMed:19287378, ECO:0000269|PubMed:21455486, ECO:0000269|PubMed:22264607, ECO:0000269|PubMed:26926603}.

Molecular Weight: 94.5 kDa

UniProt: Q9ET47

AlphaFold: Q9ET47

Pathways: Sensory Perception of Sound