CCDC22 Protein (AA 1-627) (His tag)

Catalog No. ABIN7565088

Recombinant CCDC22 protein expressed in HEK-293 Cells.

Quick Overview for CCDC22 Protein (AA 1-627) (His tag) (ABIN7565088)

Target: CCDC22 (Coiled-Coil Domain Containing 22 (CCDC22))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-627

Purification tag / Conjugate: This CCDC22 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ccdc22 Protein expressed in mammalian cells.

Sequence: MEEADRILIH SLRQAGTAVP PEVQTLRAFT TELVVEAVVR CLRVINPDVG SGLSHLLPPA MSARFRLAMS LAQACMDLGY PLELGYQNFL YPSEPDLRDL LLFLAERLPS DASEDADQPA GDSAIFLRAI GSQIRDQLAL PWVPPLLRTP KVQRLQGSAL QQPFHSSRLV LPELNSSGEL WEFQASPLLL PAPTQVPQLQ GRAASLLEHH ASQLCQHVNR DCPGDEDRVR WASRVPSQED SRAPQQRLHK QLIEHLRQSW GPLGAPTQVR DLGEMLQTWG ARAMTGVPKG SRFTHSEKFT FHLEPQVQAA QVADVPATSQ RLEQDTRAAQ EQELESLREQ LASVNHNIEE VEADMKTLGI NLVQVETECR QSELSVAEQE QALRLKSRTV ELLPDGAANL AKLQLVVESS AQRLIHLASQ WEKHRVPLLA EYRHLRRLQD CRELESSRRL AEIQELHHSV RAAAEEARRK EEVYKQLVSE LETLPKDVSR LAYTQRILEI VGNIRKQKEE ITKILSDTKE LQKEINSLSG KLDRTFAVTD ELVFKDAKKD DAVRKAYKYL AALHENCSQL IQTIEDTGTI MREVRDLEEQ IETEMGKKTL SNLEKICEDY RALRQENAGL LGRVREA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: CCDC22 (Coiled-Coil Domain Containing 22 (CCDC22))

Alternative Name: Ccdc22

Background: Coiled-coil domain-containing protein 22,FUNCTION: Involved in regulation of NF-kappa-B signaling. Promotes ubiquitination of I-kappa-B-kinase subunit IKBKB and its subsequent proteasomal degradation leading to NF-kappa-B activation, the function may involve association with COMMD8 and a CUL1-dependent E3 ubiquitin ligase complex. May down-regulate NF-kappa-B activity via association with COMMD1 and involving a CUL2-dependent E3 ubiquitin ligase complex. Regulates the cellular localization of COMM domain-containing proteins, such as COMMD1 and COMMD10. Component of the CCC complex, which is involved in the regulation of endosomal recycling of surface proteins, including integrins, signaling receptor and channels. The CCC complex associates with SNX17, retriever and WASH complexes to prevent lysosomal degradation and promote cell surface recycling of numerous cargos such as integrins ITGA5:ITGB1. Plays a role in copper ion homeostasis. Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery, the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes. {ECO:0000250|UniProtKB:O60826}.

Molecular Weight: 70.8 kDa

UniProt: Q9JIG7

AlphaFold: Q9JIG7