PIGO Protein (AA 1-1093) (His tag)

Catalog No. ABIN7565102

Product Details

Target: PIGO (Phosphatidylinositol Glycan Anchor Biosynthesis, Class O (PIGO))

Protein Type: Recombinant

Protein Characteristics: AA 1-1093

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This PIGO protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Pigo Protein expressed in mammalian cells.

Sequence: MRKVSVLLFL AWVCFLFYAG IALFTSGFLL TRLELTNQSS CQELPGPGPL PWGSHGKPGA CWMPSRFSRV VLVLIDALRF DFAQPQRSHV PGEPPVSVPF LGKLGSLQRI LESQPHHGRL YRSQVDPPTT TMQRLKALTT GSLPTFIDAG SNFASHAIVE DNVIQQLNSA GRRVVFMGDD TWRDLFPGAF SQAFFFSSFN VRDLHTVDNG ILEHLYPTLD GGSWDVLIAH FLGVDHCGHK HGPHHPEMAK KLSQMDQVIQ GLIERLENDT LLVVAGDHGM TMNGDHGGDS ELEVSAALFL YSPTALFPSV PPEEPEVIPQ VSLVPTLALL LGLPIPFGNT GEVMAELFSG GSDSSHPHSS ALAQVSALHI NAQQVSRFLH TYSAATQDLQ VKELHRLQTL FSKASARYQH FLRDPQEAEA ALSTLTAEFQ QFLRGARALC IESWARFSLV RMAGGAALLA AACLLCLLAS QLAVAPGFLF RPLLLIPVAW GLTWTILYAG VSVTTGSKID LVVLGAVAAA GSLLPFLWKA WVSRGSKRPL APLLPVPRPV LILLLIRLAT FFSDSFFVVE ARATPFLLGS LVFFLVAQLH WEGQLLPPKP LTMSRLGSSA PTAPPRHSGA HALWLGIGLL LFTRLAGLFH RCPEETPACR SSPWLSPLAS MVGGRAKNLW YGACVGALVA LLVVVRLWLR RYGNLKSPEP PVLFVRWGMP LMVLGTAAYW ALASGAEEAP PRLRALVAGA SAVLPRAVMG LAALGLVLLL WRPVTVLVKA GAATSRTRTI LTPFSGPPTS QADLDYVVPQ IYRHMQEEFQ GRLERTKAQG PITVAAYQLG SVYSAAMVTA LLLLAFPLML LHVERVSLVF LLLFLQSFLL LHLLAAGTPV ATPGPFTVLW QAVSAWVLLA TQTFYSTGHQ PVFSAIHWHA AFVGFPDGHG SSTWLPALLV GANTFASHLL FAVGCPLLLL WPFLCERQGP KRRQPLPGSE SEARVRPEEE EELQEPLMEV RLRDAPNHFN AALLQLGLKY LFILGAQILA CALAASILRR HLMVWKVFAP KFIFEAVGFI VSSVGLLLGI ALVMRVDVAV SSWFKKLVLA QQR Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PIGO (Phosphatidylinositol Glycan Anchor Biosynthesis, Class O (PIGO))

Alternative Name: Pigo (PIGO Products)

Background: GPI ethanolamine phosphate transferase 3 (EC 2.-.-.-) (Phosphatidylinositol-glycan biosynthesis class O protein) (PIG-O),FUNCTION: Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the GPI third mannose which links the GPI-anchor to the C-terminus of the proteins by an amide bond. {ECO:0000269|PubMed:10781593}.

Molecular Weight: 119.2 kDa

UniProt: Q9JJI6

AlphaFold: Q9JJI6

Pathways: Inositol Metabolic Process, SARS-CoV-2 Protein Interactome