NPHP1 Protein (AA 1-687) (His tag)

Catalog No. ABIN7565155

Product Details

Target: NPHP1 (Nephronophthisis 1 (Juvenile) (NPHP1))

Protein Type: Recombinant

Protein Characteristics: AA 1-687

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This NPHP1 protein is labelled with His tag.

Purpose: Custom-made recombinant Nphp1 Protein expressed in mammalian cells.

Sequence: MLARRPRDPL QALRRRGQEL KLQVDSLVTE SQLTGALEPS KRREIYQRCI QLKQAVDENK NTLQKLNKAD EAAPVGNYEQ RKEEEHSLLE KLACQLQELA VSISRKDALK VEAHSDKEED DTTEDDEEET GGEEEESEGD GEGKQEQASP KQAETETVTY IALGDFAAQQ TGDLTFKKGD VLHIIEKKPD GWWLAKDAEG VEGLIPRTYL EPYNKEDKLE SSEGSEEGGE EDGEEDVEVV DETADGAQVK QRTDSHWSAV RKAISEQINT VDVLATMGAI PAGFRPSTLS QLLDEAGNQF RASYFLQPEL TTSQLAFRDL TWDAKAGTIM SRPSRVSLIL TLWSCKMIPL PGTSIQVLSR HIRLCLFDGS KVLSNIHTVR AVWQPKKPKT WTFSPQVTGI LPCLLDGDCF IRSNSSTPDL GILFELGISY IRNSTGERGE LSCGWVFLKL FDASGVPIPA KTYELFLNGG TPYEKGVEVD PSVSRRAQGS VFRQMISVRR QPQLLVKLRS LNRRSRAMLS LLPETLVGSM CSAHLLIFYR QILGDVLLRD RTNLQSADLI SHPVLATFPL LLEQPDVMDA LRSSWSEKES TLKRAEKRDK ELLKAEFLLV YHDCVLPLLH STLLPPFRWA EEETEAARWK AIADFLKQSR ENEGSLKALL SPDGVHKPFD LSEQTFDFLG EIRKNSG Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NPHP1 (Nephronophthisis 1 (Juvenile) (NPHP1))

Alternative Name: Nphp1 (NPHP1 Products)

Background: Nephrocystin-1,FUNCTION: Together with BCAR1 it may play a role in the control of epithelial cell polarity. Involved in the organization of apical junctions in kidney cells, together with NPHP4 and RPGRIP1L/NPHP8 (PubMed:21565611). Does not seem to be strictly required for ciliogenesis (PubMed:19208653). Seems to help to recruit PTK2B/PYK2 to cell matrix adhesions, thereby initiating phosphorylation of PTK2B/PYK2 and PTK2B/PYK2-dependent signaling (PubMed:11493697). May play a role in the regulation of intraflagellar transport (IFT) during cilia assembly (PubMed:19208653). Required for normal retina development (PubMed:19208653). In connecting photoreceptor cilia influences the movement of some IFT proteins such as IFT88 and WDR19 (PubMed:19208653). Involved in spermatogenesis, required for the differentiation of early elongating spermatids into spermatozoa (PubMed:18684731). {ECO:0000269|PubMed:11493697, ECO:0000269|PubMed:18684731, ECO:0000269|PubMed:19208653, ECO:0000269|PubMed:21565611}.

Molecular Weight: 77.0 kDa

UniProt: Q9QY53