NUMB Protein (AA 1-653) (His tag)

Catalog No. ABIN7565170

This Recombinant NUMB protein is produced in HEK-293 Cells.

Quick Overview for NUMB Protein (AA 1-653) (His tag) (ABIN7565170)

Target: NUMB (Numb Homolog (NUMB))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-653

Purification tag / Conjugate: This NUMB protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Numb Protein expressed in mammalian cells.

Sequence: MNKLRQSFRR KKDVYVPEAS RPHQWQTDEE GVRTGKCSFP VKYLGHVEVD ESRGMHICED AVKRLKAERK FFKGFFGKTG KKAVKAVLWV SADGLRVVDE KTKDLIVDQT IEKVSFCAPD RNFDRAFSYI CRDGTTRRWI CHCFMAVKDT GERLSHAVGC AFAACLERKQ KREKECGVTA TFDASRTTFT REGSFRVTTA TEQAEREEIM KQLQDAKKAE TDKTVVGPSV APGNTAPSPS SPTSPTPDGT ASSEMNNPHA IPRRHAPIEQ LARQGSFRGF PALSQKMSPF KRQLSLRINE LPSTMQRKTD FPIKNTVPEV EGEAESISSL CSQITSAFST PSEDPFSSAP MTKPVTLVAP QSPVLQANGT DSASHVLTAK PANTALAHVA MPVRETNPWA HVPDAANKEI AAIHPGTEWG QSSGAASPGL FQAGHRRTPS EADRWLEEVS KSVRAQQPQV SAAPLQPVLQ PPPPAAIAPP APPFQGHAFL TSQPVPVGVV PPLQPAFVPT QSYPVANGMP YPASNVPVVG ITPSQMVANV FGTAGHPQTT HPHQSPSLAK QQTFPQYETS SATTSPFFKP PAQHLNGSAA FNGVDNGGLA SGNRHAEVPP GTCPVDPFEA QWAALESKSK QRTNPSPTNP FSSDLQKTFE IEL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NUMB (Numb Homolog (NUMB))

Alternative Name: Numb

Background: Protein numb homolog (m-Nb) (m-Numb),FUNCTION: Regulates clathrin-mediated receptor endocytosis (By similarity). Plays a role in the process of neurogenesis (PubMed:10841580, PubMed:12410312, PubMed:15273690, PubMed:17174898). Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate (PubMed:15273690, PubMed:12410312). Not required for the proliferation of neural progenitor cells before the onset of neurogenesis (PubMed:15273690, PubMed:12410312). Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity (PubMed:17174898). May also mediate local repair of brain ventricular wall damage (PubMed:17174898). {ECO:0000250|UniProtKB:P49757, ECO:0000269|PubMed:10841580, ECO:0000269|PubMed:12410312, ECO:0000269|PubMed:15273690, ECO:0000269|PubMed:17174898}.

Molecular Weight: 70.8 kDa

UniProt: Q9QZS3

AlphaFold: Q9QZS3

Pathways: Cell-Cell Junction Organization