Naip5 Protein (AA 1-1403) (His tag)

Catalog No. ABIN7565172

Recombinant Naip5 protein expressed in HEK-293 Cells.

Quick Overview for Naip5 Protein (AA 1-1403) (His tag) (ABIN7565172)

Target: Naip5 (NAIP5) (NLR Family, Apoptosis Inhibitory Protein 5 (NAIP5))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1403

Purification tag / Conjugate: This Naip5 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Naip5 Protein expressed in mammalian cells.

Sequence: MAEHGESSED RISEIDYEFL PELSALLGVD AFQVAKSQEE EEHKERMKMK KGFNSQMRSE AKRLKTFETY DTFRSWTPQE MAAAGFYHTG VRLGVQCFCC SLILFGNSLR KLPIERHKKL RPECEFLQGK DVGNIGKYDI RVKRPEKMLR GGKARYHEEE ARLESFEDWP FYAHGTSPRV LSAAGFVFTG KRDTVQCFSC GGSLGNWEEG DDPWKEHAKW FPKCEFLQSK KSSEEIAQYI QSYEGFVHVT GEHFVKSWVR RELPMVSAYC NDSVFANEEL RMDMFKDWPQ ESPVGVEALV RAGFFYTGKK DIVRCFSCGG CLEKWAEGDD PMEDHIKFFP ECVFLQTLKS SAEVIPTLQS QYALPEATET TRESNHGDAA AVHSTVVDLG RSEAQWFQEA RSLSEQLRDN YTKATFRHMN LPEVCSSLGT DHLLSCDVSI ISKHISQPVQ EALTIPEVFS NLNSVMCVEG ETGSGKTTFL KRIAFLWASG CCPLLYRFQL VFYLSLSSIT PDQGLANIIC AQLLGAGGCI SEVCLSSSIQ QLQHQVLFLL DDYSGLASLP QALHTLITKN YLSRTCLLIA VHTNRVRDIR LYLGTSLEIQ EFPFYNTVSV LRKFFSHDII CVEKLIIYFI DNKDLQGVYK TPLFVAAVCT DWIQNASAQD KFQDVTLFQS YMQYLSLKYK ATAEPLQATV SSCGQLALTG LFSSCFEFNS DDLAEAGVDE DEKLTTLLMS KFTAQRLRPV YRFLGPLFQE FLAAVRLTEL LSSDRQEDQD LGLYYLRQID SPLKAINSFN IFLYYVSSHS SSKAAPTVVS HLLQLVDEKE SLENMSENED YMKLHPQTFL WFQFVRGLWL VSPESSSSFV SEHLLRLALI FAYESNTVAE CSPFILQFLR GKTLALRVLN LQYFRDHPES LLLLRSLKVS INGNKMSSYV DYSFKTYFEN LQPPAIDEEY TSAFEHISEW RRNFAQDEEI IKNYENIRPR ALPDISEGYW KLSPKPCKIP KLEVQVNNTD AADQALLQVL MEVFSASQSI EFRLFNSSGF LESICPALEL SKASVTKCSM SRLELSRAEQ ELLLTLPALQ SLEVSETNQL PEQLFHNLHK FLGLKELCVR LDGKPNVLSV LPREFPNLLH MEKLSIQTST ESDLSKLVKF IQNFPNLHVF HLKCDFLSNC ESLMAVLASC KKLREIEFSG RCFEAMTFVN ILPNFVSLKI LNLKDQQFPD KETSEKFAQA LGSLRNLEEL LVPTGDGIHQ VAKLIVRQCL QLPCLRVLTF HDILDDDSVI EIARAATSGG FQKLENLDIS MNHKITEEGY RNFFQALDNL PNLQELNICR NIPGRIQVQA TTVKALGQCV SRLPSLIRLH MLSWLLDEED MKVINDVKER HPQSKRLIIF WKLIVPFSPV ILE Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Naip5 (NAIP5) (NLR Family, Apoptosis Inhibitory Protein 5 (NAIP5))

Alternative Name: Naip5

Background: Baculoviral IAP repeat-containing protein 1e (Neuronal apoptosis inhibitory protein 5),FUNCTION: Sensor component of the NLRC4 inflammasome that specifically recognizes and binds flagellin from pathogenic bacteria such as Legionella or Salmonella (PubMed:12526741, PubMed:21874021, PubMed:21918512, PubMed:29146805, PubMed:29182158). Association of pathogenic bacteria proteins drives in turn drive assembly and activation of the NLRC4 inflammasome, promoting caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:21874021, PubMed:21918512, PubMed:29146805, PubMed:29182158). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri (PubMed:21874021, PubMed:21918512, PubMed:29146805, PubMed:29182158). May contribute to prevent motor-neuron apoptosis induced by a variety of signals (By similarity). {ECO:0000250|UniProtKB:Q13075, ECO:0000269|PubMed:12526741, ECO:0000269|PubMed:21874021, ECO:0000269|PubMed:21918512, ECO:0000269|PubMed:29146805, ECO:0000269|PubMed:29182158}.

Molecular Weight: 159.8 kDa

UniProt: Q9R016

AlphaFold: Q9R016