AP3B1 Protein (AA 1-1105) (His tag)

Catalog No. ABIN7565283

Product Details

Target: AP3B1 (Adaptor-Related Protein Complex 3, beta 1 Subunit (AP3B1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1105

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This AP3B1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ap3b1 Protein expressed in mammalian cells.

Sequence: MSSNSFAYNE QSGGGEAAEL GQEATSTISP SGAFGLFSSD WKKNEDLKQM LESNKDSAKL DAMKRIVGMI AKGKNASELF PAVVKNVASK NIEIKKLVYV YLVRYAEEQQ DLALLSISTF QRALKDPNQL IRASALRVLS SIRVPIIVPV MMLAIKEASA DLSPYVRKNA AHAIQKLYSL DPEQKEMLIE VIEKLLKDKS TLVAGSVVMA FEEVCPDRID LIHRNYRKLC NLLVDVEEWG QVVIIHMLTR YARTQFVSPW REDGGLEDNE KNFYESEEEE EEKEKSSRKK SYAMDPDHRL LIRNTKPLLQ SRNAAVVMAV AQLYWHISPK SEAGVISKSL VRLLRSNREV QYIVLQNIAT MSIERKGMFE PYLKSFYVRS TDPTMIKTLK LEILTNLANE ANISTLLREF QTYVRSQDKQ FAAATIQTIG RCATSISEVT DTCLNGLVCL LSNRDEIVVA ESVVVIKKLL QMQPAQHGEI IRHMAKLLDS ITVPVARASI LWLIGENCER VPKIAPDVLR KMAKSFTSED DLVKLQILNL AAKLYLTNSK QTKLLTQYIL NLGKYDQNYD IRDRTRFIRQ LIVPNEKSGA LSKYAKKIFL APKPAPLLES PFKDRDRFQL GTLSHTLNIK ASGYLELSNW PEVAPDPSVR NVEVIESAKE WTPLGKTKKE KPMKKFYSES EEEEDEDEDE DEEEEEKEDE DENPSDSSSD SESGSGSESG DTGTEDSSED SSSGQDSETG SQAEAERQKV AKRNSKTKRK SDSENREKKN ENSKASESSS EESSSMEDSS SESESESGSD SEPAPRNVAP AKERKPQQER HPPSKDVFLL DLDDFNPVST PVALPTPALS PSLIADLEGL NLSTSSSVIN VSTPVFVPTK THELLHRMHG KGLAAHYCFP RQPCIFSDKM VSVQITLTNT SDRKIENIHI GGKGLPVGMQ MHAFHPIDSL EPKGSVTVSV GIDFCDSTQT ASFQLCTKDD CFNVTLQPPV GELLSPVAMS EKDFKKEQGT LTGMNETSAT LIAAPQNFTP SMILQKVVNV ANLGAVPSSQ DNVHRFAART VHSGSLMLVT VELKEGSTAQ LIINTEKTVI GSVLLRELKP VLSQG Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: AP3B1 (Adaptor-Related Protein Complex 3, beta 1 Subunit (AP3B1))

Alternative Name: Ap3b1 (AP3B1 Products)

Background: AP-3 complex subunit beta-1 (Adaptor protein complex AP-3 subunit beta-1) (Adaptor-related protein complex 3 subunit beta-1) (Beta-3A-adaptin) (Clathrin assembly protein complex 3 beta-1 large chain),FUNCTION: Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. {ECO:0000269|PubMed:21998198}.

Molecular Weight: 122.7 kDa

UniProt: Q9Z1T1

AlphaFold: Q9Z1T1

Pathways: SARS-CoV-2 Protein Interactome