TIMD4 Protein (AA 25-315) (Fc Tag)

Catalog No. ABIN7566381

This Recombinant TIMD4 protein is expressed in HEK-293 Cells.

Quick Overview for TIMD4 Protein (AA 25-315) (Fc Tag) (ABIN7566381)

Target: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >98 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 25-315

Purification tag / Conjugate: This TIMD4 protein is labelled with Fc Tag.

Purpose: Tim-4 (human):Fc (mouse) (rec.)

Cross-Reactivity: Human

Characteristics: The extracellular domain of human Tim-4 (aa 25-315) is fused to the N-terminus of the Fc region of mouse IgG2a.

Sterility: Sterile filtered

Endotoxin Level: <5EU/mg protein (LAL test, Lonza).

Biological Activity Comment: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Alternative Name: Tim-4

Background:

TIM4, TIMD4, T Cell Immunoglobulin and Mucin Domain-containing Protein 4, T Cell Membrane Protein 4

The TIM (T cell/transmembrane, immunoglobulin and mucin) family plays a critical role in regulating immune responses, including allergy, asthma, transplant tolerance, autoimmunity and the response to viral infections. The unique structure of TIM immunoglobulin variable region domains allows highly specific recognition of phosphatidylserine (PtdSer), exposed on the surface of apoptotic cells. TIM-4 (T cell, immunoglobulin, Mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIM-4 is exclusively expressed on antigen-presenting cells, where it mediates phagocytosis of apoptotic cells and plays an important role in maintaining tolerance. TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma. Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells. The interaction of TIM-4 with TIM-1 induces costimulatory and hyperproliferative signals in T cells.

Molecular Weight: ~80kDa (SDS-PAGE)

NCBI Accession: NP_612388

Pathways: Cancer Immune Checkpoints