BDS-II Protein

Catalog No. ABIN7821898

This Native BDS-II protein is expressed in Sea Anemone.

Quick Overview for BDS-II Protein (ABIN7821898)

Target: BDS-II

Protein Type: Native

Origin: Anemonia sulcata

Source: Sea Anemone

Application: Blocking Peptide (BP)

Purity: >95 % (HPLC)

Product Details

Purpose: A Blocker of KV3 Channels and a Modulator of NaV Channels

Sequence: AAPCFCPGKP DRGDLWILRG TCPGGYGYTS NCYKWPNICC YPH-OH

Specificity: Toxin target: KV3 K+ channels, NaV Na+ channels,
Disulfide bonds between: Cys4-Cys39, Cys6-Cys32, and Cys22-Cys40

Characteristics: A Blocker of KV3 Channels and a Modulator of NaV Channels

Application Details

Application Notes: Antigen Preadsorption Control: 100 nM - 5 μM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Buffer: Lyophilized from double distilled water (ddH2O).

Storage: 4 °C,-20 °C

Storage Comment: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Target Details

Target: BDS-II

Background: DeltaKappa-actitoxin-Avd4b, Blood depressing substance II, Blood depressing substance 2,BDS-II is a 43 amino acid peptidyl toxin isolated from the sea anemone Anemonia sulcata venom. BDS-II was shown to be a specific KV3.4 blocker. BDS-II blocked 70 % of the KV3.4 current in COS-transfected cells at a concentration of 2.8 μM The blocking effect was rapid, direct and reversible1. Recently it was shown that BDS-II blocks other KV3 channels with similar potencies.2The closely related BDS-I was shown to modulate voltage-gated Na+ channels. It enhanced TTX-sensitive Na+ channels (highly effective on NaV1.7 channels), and weakly inhibited TTX-resistant NaV channels3. As such, we showed that BDS-II also potently inhibits NaV1.7 channels (see Our Bioassay).

Molecular Weight: 4776.4 Da

UniProt: P59084