delta-Dendrotoxin Protein

Catalog No. ABIN7821949

Native delta-Dendrotoxin protein expressed in Snake (Serpentes).

Quick Overview for delta-Dendrotoxin Protein (ABIN7821949)

Target: delta-Dendrotoxin

Protein Type: Native

Origin: Dendroaspis angusticeps

Source: Snake (Serpentes)

Application: Blocking Peptide (BP)

Purity: ≥98 % (HPLC)

Product Details

Purpose: A Potent and Specific Blocker of KV1.1 Channels and a Less Potent Blocker of Kir1.1 (ROMK1) Channels

Sequence: AAKYCKLPVR YGPCKKKIPS FYYKWKAKQC LPFDYSGCGG NANRFKTIEE CRRTCVG-OH

Specificity: Toxin target: KV1.1, KV1.6 and Kir1.1 K+ channels,
Disulfide bonds between: Cys5-Cys55, Cys14-Cys38 and Cys30-Cys51

Characteristics: A Potent and Specific Blocker of KV1.1 Channels and a Less Potent Blocker of Kir1.1 (ROMK1) Channels

Application Details

Application Notes: Antigen Preadsorption Control: 10 - 100 nM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage: 4 °C,-20 °C

Storage Comment: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Target Details

Target: delta-Dendrotoxin

Background: δ-Dendrotoxin is isolated from Dendroaspis angusticeps1 snake venom by modification of the procedures of Harvey2 and Benishin1 and purified to homogeneity.δ-Dendrotoxin blocks KV1.1 channels (IC50 = 0.1 nM,3 for review see 4). The toxin was shown to also block KV1.6 (IC50= 23 nM).4 In addition δ-Dendrotoxin blocks ROMK1 inward rectifier K+ channels with Kd of 150 nM3

Molecular Weight: 6568 Da

UniProt: P00982