Dendrotoxin-I Protein

Catalog No. ABIN7821952

This Native Dendrotoxin-I protein is produced in Snake (Serpentes).

Quick Overview for Dendrotoxin-I Protein (ABIN7821952)

Target: Dendrotoxin-I

Protein Type: Native

Origin: Dendraspis polylepis polylepis

Source: Snake (Serpentes)

Application: Blocking Peptide (BP)

Purity: ≥98 % (HPLC)

Product Details

Purpose: A Potent Blocker of KV1.1 and KV1.2 Channels

Sequence: QPLRKLCILH RNPGRCYQKI PAFYYNQKKK QCEGFTWSGC GGNSNRFKTI EECRRTCIRK -OH

Specificity: Toxin target: KV1.1, KV1.2 K+ channels,
Disulfide bonds between: Cys7-Cys57, Cys16-Cys40, and Cys32-Cys53

Characteristics: A Potent Blocker of KV1.1 and KV1.2 Channels

Application Details

Application Notes: Antigen Preadsorption Control: 10 - 100 nM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage: 4 °C,-20 °C

Storage Comment: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Target Details

Target: Dendrotoxin-I

Background: Venom basic protease inhibitor 1, Dendrotoxin-1, DTX-I, DTX-1,Dendrotoxin-I is isolated from Dendroaspis p.polylepis snake venom by modification of the procedure of Schweitz1 and purified to homogeneity.Dendrotoxin-I blocks KV1.2 and KV1.1 channels (IC50=0.13 and 3.1 nM in oocytes respectively, with higher values for mammalian cells)3 as well as heteromultimeric channels containing these, with other KV1 isoforms2 (for review see 3).

Molecular Weight: 7149 Da

UniProt: P00979